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首页> 外文会议>Advances in chromosome sciences >Image analysis of plant chromosomes by using CHIAS III
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Image analysis of plant chromosomes by using CHIAS III

机译：利用CHIAS III对植物染色体进行图像分析

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The third-generation chromosome image analyzing system (CHIAS III) was developed using an ordinary personal computer with public domain imaging software, NIH Image, Uneven condensation patterns (CP) appearing on small chromosomes at the mitotic prometaphase stage were analyzed. A quantitative chromosome map or idiogram was developed by the CHIAS III. The analysis consists of three major steps as follows: ①Manipulation of original chromosome images and measurement CP, ②calculation of an averaged CP of homologous chromosomes, and ③development an idiogram based on the averaged CP. The CPs had been analyzed quantitatively by using the first and the second-generation CHIAS with the hard and software specific to the systems . The data obtained using CHIAS III are consistent with those obtained with the chromosome image analyzing systems previously developed. CHIAS III is opened to the public on the Internet ( http://133.1.131.81/Eudejas/chias3/Chias3. html). Quantitative chromosome maps based on the CP Using CHIAS III have been developed in several plant species, for example Oryza sativa, Arabidopsis thaliana, Saccharum spontaneum, Spinacia oleracea, etc. Other applications have also been developed through the CHIAS analysis of FISH signals, idiogram viewer by JAVA, pachytene version. It is anticipated that the development of CHIAS and stepwise new applications to the public will greatly contribute for quantitative analysis of chromosome information.
机译：使用具有公共领域成像软件NIH Image的普通个人计算机开发了第三代染色体图像分析系统（CHIAS III），分析了在有丝分裂前中期阶段出现在小染色体上的不均匀冷凝模式（CP）。 CHIAS III开发了定量染色体图或独特图。分析包括以下三个主要步骤：①操纵原始染色体图像并测量CP；②计算同源染色体的平均CP；③根据平均CP绘制图状图。通过使用第一代和第二代CHIAS以及针对系统的软件和硬件对CP进行了定量分析。使用CHIAS III获得的数据与以前开发的染色体图像分析系统获得的数据一致。 CHIAS III在Internet（http://133.1.131.81/Eudejas/chias3/Chias3.html）上向公众开放。基于CHIAS III CP的定量染色体图谱已经在几种植物中得到开发，例如水稻，拟南芥，自发糖蔗，菠菜等。通过对FISH信号的CHIAS分析，本位图查看器，还开发了其他应用。由JAVA，粗线版本。可以预料，CHIAS的发展和逐步向公众推广的新应用将大大有助于染色体信息的定量分析。

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