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Analyses of Progenies and Their Parents for Zea mays x Zea diploperennis Using GISH

机译:利用GISH分析玉米和玉米的后代及其亲本

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摘要

In this study, Zea diploperennis (DP) introgressed segments in genomes of the tested maize lines were detected by both southern hybridization and genomic in situ hybridization (GISH) . In addition, the probes of the genomic DNAs, Cot-1 DNAs and non-Cot-1 DNAs in maize Lu 9 and DP were applied for comparative fluorescence in situ hybridization (FISH) with chromosomes of maize inbred line Lu 9 and DP.
机译:在该研究中,通过Southern杂交和基因组原位杂交(GISH)都检测了被测玉米品系的基因组中的Zea diploperennis(DP)渗入片段。此外,将玉米Lu 9和DP中的基因组DNA,Cot-1 DNA和非Cot-1 DNA探针用于与玉米自交系Lu 9和DP染色体进行的比较荧光原位杂交(FISH)。

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