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首页> 外文会议>Advances in structures, properties and applications of biological and bioinspired materials >Proliferation and Osteogenic Differentiation of Mesenchymal Stem Cells on Biodegradable Calcium-deficient Hydroxyapatite Tubular Bacterial Cellulose Composites
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Proliferation and Osteogenic Differentiation of Mesenchymal Stem Cells on Biodegradable Calcium-deficient Hydroxyapatite Tubular Bacterial Cellulose Composites

机译：可生物降解缺钙的羟基磷灰石管状细菌纤维素复合材料间充质干细胞的增殖和成骨分化。

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Advanced biomaterials that mimic the structure and function of native tissues and permit stem cells to adhere and differentiate is of paramount importance in the development of stem cell therapies for bone defects. Successful bone repair approaches may include an osteoconductive scaffold that permits excellent cell adhesion and proliferation, and cells with an osteogenic potential. The objective of this study was to evaluate the cell proliferation, viability and osteocyte differentiation of equine-derived bone marrow mesenchymal stem cells (EqMSCs) when seeded onto biocompatible and biodegradable calcium-deficient hydroxyapatite (CdHA) tubular-shaped bacterial cellulose scaffolds (BC-TS) of various sizes. The biocompatible gel-like BC-TS was synthesized using the bacterium Gluconacetobacter sucrofermentans under static culture in oxygen-permeable silicone tubes. The BC-TS scaffolds were modified using a periodate oxidation to yield biodegradable scaffolds. Additionally, CdHA was deposited in the scaffolds to mimic native bone tissues. The morphological properties of the resulting BC-TS and its composites were characterized using scanning electron microscopy. The ability of the BC-TS and its composites to support and maintain EqMSCs growth, proliferation and osteogenic differentiation in vitro was also assessed. BC-TS and its composites exhibited aligned nanofibril structures. MTS assay demonstrated increasing proliferation and viability with time (days 1, 2 and 3). Cell-scaffold constructs were cultured for 8 days under osteogenic conditions and the resulting osteocytes were positive for alizarin red. In summary, biocompatible and biodegradable CdHA BC-TS composites support the proliferation, viability and osteogenic differentiation of EqMSCs cultured onto its surface in vitro, allowing for future potential use for tissue engineering therapies.
机译：模仿天然组织的结构和功能并允许干细胞粘附和分化的先进生物材料在开发用于骨缺损的干细胞疗法中至关重要。成功的骨修复方法可能包括骨传导支架，该支架允许出色的细胞粘附和增殖，以及具有成骨潜能的细胞。这项研究的目的是评估马源骨髓间充质干细胞（EqMSCs）植入生物相容且可生物降解的钙缺乏性羟基磷灰石（CdHA）管状细菌纤维素支架（BC-）时的细胞增殖，活力和骨细胞分化TS）。生物相容性凝胶状BC-TS是用细菌葡糖糖杆菌（Gluconacetobacter sucrofermentans）在透氧有机硅管中静态培养下合成的。使用高碘酸盐氧化修饰BC-TS支架以产生可生物降解的支架。另外，将CdHA沉积在支架中以模拟天然骨组织。使用扫描电子显微镜表征所得BC-TS及其复合物的形态学特性。还评估了BC-TS及其复合物在体外支持和维持EqMSCs生长，增殖和成骨分化的能力。 BC-TS及其复合材料表现出对齐的纳米原纤维结构。 MTS分析显示随着时间（第1、2和3天）增殖和生存能力的增加。在成骨条件下将细胞支架构建物培养8天，所得骨细胞的茜素红呈阳性。总之，生物相容性和可生物降解的CdHA BC-TS复合材料支持体外培养在其表面上的EqMSC的增殖，生存力和成骨分化，从而为组织工程疗法的未来使用提供了可能。

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