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Profiling Expression Patterns and Isolating Differentially Expressed Genes by cDNA Microarray System with Colorimetry Detection

机译：用比色法检测cDNA微阵列系统分析表达模式并分离差异表达基因

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摘要

A high-density cDNA microarray with colorimetry detection system to simultaneously monitor the expression of many genes on nylon membrane is described and characterized. To quantify the expression of genes and to isolate differentially expressed genes, the southern hybridization process on filter membranes was employed. The levels of gene expression were represented by color intensities generated by colorimetric reactions in place of hazardous radioiso-topes or costly laser-induced fluorescence detection. The gene expression patterns on nylon membranes were digitized by devices such as an economical flatbed scanner or a digital camera. The quantitative information of gene expression was retrieved by image analysis software. Quantitative comparison of the northern dot-blotting method with the microarray system is described. Applications employing single-color detection as well as dual-color detection to isolate differentially expressed genes among thousands of genes are demonstrated.
机译：描述并表征了具有比色检测系统的高密度cDNA微阵列，可同时监视尼龙膜上许多基因的表达。为了量化基因的表达并分离差异表达的基因，采用了在滤膜上的Southern杂交方法。基因表达水平用比色反应产生的颜色强度代替危险的放射性同位素或昂贵的激光诱导荧光检测来表示。尼龙膜上的基因表达模式通过经济型平板扫描仪或数码相机等设备进行数字化处理。通过图像分析软件检索基因表达的定量信息。描述了Northern点印迹法与微阵列系统的定量比较。展示了采用单色检测和双色检测在数千个基因中分离差异表达基因的应用。

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