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首页> 外文会议>Biofabrication for hierarchical in vitro tissue models >CHONDROGENIC POTENTIAL OF CHONDROCYTES IN HYALURONIC ACID/PEG-BASED HYDROGELS IS DEPENDENT ON THE HYALURONIC ACID CONCENTRATION
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CHONDROGENIC POTENTIAL OF CHONDROCYTES IN HYALURONIC ACID/PEG-BASED HYDROGELS IS DEPENDENT ON THE HYALURONIC ACID CONCENTRATION

机译：透明质酸/ PEG基水合物中软骨细胞的软骨电位与透明质酸浓度有关

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Purpose: Hydrogels based on PEG and methacrylated poly(N-(2-hydroxypropyl) methacrylamide-mono/dilactate) (M_(10)P_(10)) are promising biomaterials for Biofabrication of cartilage constructs. Addition of hyaluronic acid (HA) to a hydrogel improves printability by increasing the viscosity. Methacrylating HA (HAMA) can ensure covalent binding in M_(10)P_(10) hydrogels after UV-cross-linking. Chondrocytes can interact with HAMA via their CD44 receptor, however, the influence of HAMA on chondrogenic potential is unclear. This study aimed to evaluate the influence of different HAMA concentrations on chondrogenesis of chondrocytes in M10P10/HAMA hydrogels. Materials & Methods: Equine chondrocytes were encapsulated in M_(10)P_(10) hydrogels containing different HAMA concentrations. Cylindrical constructs were cast, UV-cross-linked, and cultured in TGF-β-containing medium. Constructs were analyzed for evidence of cartilage formation. Results: Preliminary data showed an increase in glycosaminoglycan (GAG)/DNA for constructs with low HAMA concentrations (0.1-0.25%) while no differences were found for higher HAMA concentrations, compared to hydrogels without HAMA (Figure 1a). Further, constructs without or with low HAMA concentrations (0.1-0.5%) demonstrated collagen type II positive areas, while this was less pronounced in constructs with 0.5-1% HAMA (n=3, Figure 1b). Conclusion: Preliminary results indicate a dose-dependent effect of HAMA on chondrogenesis of chondrocytes: low concentrations (0.1-0.25%) increase GAG production while higher concentrations (0.5-1%) have no effect on GAG production and reduce collagen type II synthesis. Ongoing evaluations will reveal the extent of chondrogenesis and its association with HAMA concentrations in M10P10/HAMA, and the mechanism responsible for the dose-dependent effect. This study will impact the use of HAMA as viscosity enhancer to improve the printability of hydrogels.
机译：目的：基于PEG和甲基丙烯酸化聚（N-（2-羟丙基）甲基丙烯酰胺-单/乳酸）（M_（10）P_（10））的水凝胶是用于软骨构造生物制备的有前途的生物材料。向水凝胶中加入透明质酸（HA）可通过增加粘度来改善可印刷性。甲基丙烯酸HA（HAMA）可确保UV交联后在M_（10）P_（10）水凝胶中共价结合。软骨细胞可以通过其CD44受体与HAMA相互作用，但是，HAMA对软骨形成潜能的影响尚不清楚。这项研究旨在评估不同的HAMA浓度对M10P10 / HAMA水凝胶中软骨细胞软骨形成的影响。材料与方法：将马软骨细胞封装在含有不同HAMA浓度的M_（10）P_（10）水凝胶中。将圆柱形构建体浇铸，UV交联并在含TGF-β的培养基中培养。分析构建体的软骨形成证据。结果：初步数据显示，与不含HAMA的水凝胶相比，具有低HAMA浓度（0.1-0.25％）的构建体的糖胺聚糖（GAG）/ DNA有所增加，而较高的HAMA浓度则没有差异（图1a）。此外，无或具有低HAMA浓度（0.1-0.5％）的构建体表现出II型胶原阳性区域，而在具有0.5-1％HAMA的构建体中则不那么明显（n = 3，图1b）。结论：初步结果表明，HAMA对软骨细胞软骨形成具有剂量依赖性：低浓度（0.1-0.25％）可增加GAG的产生，而较高浓度（0.5-1％）对GAG的产生无影响并减少II型胶原的合成。正在进行的评估将揭示软骨形成的程度及其与M10P10 / HAMA中HAMA浓度的关系，以及引起剂量依赖性效应的机制。这项研究将影响HAMA作为增粘剂的使用，以改善水凝胶的可印刷性。

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