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首页> 外文会议>Biomedical Imaging: Reporters, Dyes, and Instrumentation >Transferrin-mediated tumor delivery of contrast media for optical imaging and magnetic resonance imaging
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Transferrin-mediated tumor delivery of contrast media for optical imaging and magnetic resonance imaging

机译：转铁蛋白介导的造影剂肿瘤递送，用于光学成像和磁共振成像

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Abstract: Many tumor cells are characterized by the overexpression of highly specific transferrin receptors. Transferrin is bound to its receptor, which is followed by rapid internalization into intracellular compartments; it can thus be used for the specific delivery of coupled contrast agents in to tumor cells. The aim of our study was to investigate the tumor- specificity of transferrin-modified contrast agents for optical imaging and magnetic resonance imaging (MRI). Indotricarbocyanine, a near-IR absorbing dye, and ultrasmall superparamagnetic iron oxide particles, a contrast material for MRI, were covalently coupled to transferrin and examined in vitro and in vivo for their potential as tumor-specific contrast agents. CEll culture experiments using HT29 and A431 tumor cells showed specific uptake of transferrin- modified contrast agents by the cells at 37 degrees C, while uptake was inhibited at 4 degrees C or in the presence of an excess of unlabeled transferrin. Optical imaging of tumor- bearing nude mice after intravenous injection of transferrin-ICC resulted in pronounced tumor fluorescence. Tumor specificity was also observe in MRI, where transferrin-USPIO accumulated in SMT2A tumor-bearing rats. We conclude that transferrin-modified contrast agents for optical imaging and MRI can be specifically delivered to tumor tissue by use of transferrin receptor pathways. !13
机译：摘要：许多肿瘤细胞的特征是高特异性转铁蛋白受体的过度表达。转铁蛋白与其受体结合，随后迅速内化到细胞内区室中。因此，它可以用于将偶联的造影剂特异性递送到肿瘤细胞中。我们研究的目的是研究转铁蛋白修饰的造影剂在光学成像和磁共振成像（MRI）中的肿瘤特异性。将吲哚三氯花青素（一种近红外吸收染料）和超小超顺磁性氧化铁颗粒（用于MRI的造影剂）共价偶联至运铁蛋白，并在体内和体外检查其作为肿瘤特异性造影剂的潜力。使用HT29和A431肿瘤细胞的CEll培养实验显示，细胞在37摄氏度下特异性摄取转铁蛋白修饰的造影剂，而在4摄氏度或存在过量未标记转铁蛋白的情况下抑制摄取。静脉注射转铁蛋白-ICC后，荷瘤裸鼠的光学成像导致明显的肿瘤荧光。在MRI中也观察到了肿瘤特异性，其中运铁蛋白-USPIO蓄积在SMT2A荷瘤大鼠中。我们得出结论，可以通过使用转铁蛋白受体途径将用于光学成像和MRI的转铁蛋白修饰的造影剂特异性地递送至肿瘤组织。 ！13

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