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首页> 外文会议>Conference on biomedical imaging: Reporters, dyes, and instrumentation >Transferrin-mediated tumor delivery of contrast media for optical imaging and magnetic resonance imaging
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Transferrin-mediated tumor delivery of contrast media for optical imaging and magnetic resonance imaging

机译：转铁蛋白介导的肿瘤递送光学成像和磁共振成像

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Many tumor cells are characterized by the overexpression of highly specific transferrin receptors. Transferrin is bound to its receptor, which is followed by rapid internalization into intracellular compartments; it can thus be used for the specific delivery of coupled contrast agents in to tumor cells. The aim of our study was to investigate the tumor- specificity of transferrin-modified contrast agents for optical imaging and magnetic resonance imaging (MRI). Indotricarbocyanine, a near-IR absorbing dye, and ultrasmall superparamagnetic iron oxide particles, a contrast material for MRI, were covalently coupled to transferrin and examined in vitro and in vivo for their potential as tumor-specific contrast agents. CEll culture experiments using HT29 and A431 tumor cells showed specific uptake of transferrin- modified contrast agents by the cells at 37 degrees C, while uptake was inhibited at 4 degrees C or in the presence of an excess of unlabeled transferrin. Optical imaging of tumor- bearing nude mice after intravenous injection of transferrin-ICC resulted in pronounced tumor fluorescence. Tumor specificity was also observe in MRI, where transferrin-USPIO accumulated in SMT2A tumor-bearing rats. We conclude that transferrin-modified contrast agents for optical imaging and MRI can be specifically delivered to tumor tissue by use of transferrin receptor pathways.
机译：许多肿瘤细胞通过高度特异性的转铁蛋白受体的过度表达为特征。转铁蛋白结合到它的受体，随后是快速内在化到细胞内区室;因此它可以被用于耦合的造影剂在肿瘤细胞中的特异性递送。我们研究的目的是调查的光学成像和磁共振成像（MRI），转铁蛋白修饰的造影剂的肿瘤特异性。三羰花青，近IR吸收染料，和超小超顺磁性氧化铁颗粒，MRI造影材料，共价偶联到转铁蛋白和在体外和体内检查它们作为肿瘤特异性造影剂的潜力。使用HT29和A431肿瘤细胞的细胞培养实验显示通过细胞转铁改性的造影剂的特异性摄取在37摄氏度，而摄取在4摄氏度或在过量的未标记的转铁蛋白的存在的抑制。转铁蛋白 - ICC的静脉注射后肿瘤的裸鼠的光学成像导致显着的肿瘤荧光。肿瘤特异性也观察MRI，其中转-USPIO在SMT2A荷瘤鼠积累。我们的结论是用于光学成像和MRI转铁蛋白 - 改性的造影剂可通过使用运铁蛋白受体途径的待特异性递送到肿瘤组织。

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