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Analysis of virus infected cell by Raman spectroscopy and transmission electron microscopy

机译:拉曼光谱和透射电镜分析病毒感染的细胞

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Raman spectroscopy is a promising tool for detection of virus infection in live cells. In the present study, we demonstrate its feasibility to observe dynamic reaction of the live cell infected by virus. The Raman spectra of the adenovirus infected live cell (293 HEK) are analyzed by comparing with those of control cells. Principal component analysis (PCA) is employed also to analyze the spectra in detail. A band at 1650 cm-1 increases its intensity in the spectra measured at 24 hours after the virus infection. The infection of the virus is also examined by immune-staining and transmission electron microscope (TEM), and the virus infection is confirmed with these method also. It should be noted that the present technique does not require specifying the type of virus in advance.
机译:拉曼光谱法是检测活细胞中病毒感染的有前途的工具。在本研究中,我们证明了其观察病毒感染的活细胞动态反应的可行性。通过与对照细胞比较,分析了腺病毒感染的活细胞(293 HEK)的拉曼光谱。主成分分析(PCA)也用于详细分析光谱。在病毒感染后24小时测得的光谱中,强度为1650 cm-1的谱带增加了强度。还通过免疫染色和透射电子显微镜(TEM)检查病毒的感染,并且也通过这些方法确认了病毒感染。应当注意,本技术不需要预先指定病毒的类型。

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