• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>Biophotonics and immune responses IV >HSP70 inhibits Bax translocation during Photofrin-PDT apoptosis
【24h】

HSP70 inhibits Bax translocation during Photofrin-PDT apoptosis

机译：HSP70在Photofrin-PDT细胞凋亡期间抑制Bax易位

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Apoptosis is an important cellular event that plays a key role in therapy of many diseases. The mechanisms of the initiation and regulation of photodynamic therapy (PDT) -induced apoptosis is complex. Some PDT-associated apoptosis pathways involved plasma membrane death receptors, mitochondria, lysosomes and endoplasmic reticulum (ER). Our previous study found that Photofrin were localized primarily in mitochondria, the primary targets of Photofrin-PDT. The key role of Bax in the mitochondrion-mediated apoptosis has been demonstrated in many systems. In order to determine the role of Bax in the mitochondrion-mediated apoptosis induced by Photofrin-PDT, we used the CFP/GFP-Bax plasmid to monitor the dynamics of Bax activation and translocation after PDT treatment. With laser scanning confocal microscopy, we found that PDT induced Bax translocation from the cytosol to mitochondria; however, with cells over-expressing YFP-HSP70 plasmids, Bax translocation was not detected. Thus, for Photofrin-PDT, Bax activation and translocation were inhibited by HSP70, not influence the cell death.
机译：凋亡是重要的细胞事件，在许多疾病的治疗中起关键作用。光动力疗法（PDT）诱导的细胞凋亡的启动和调节机制很复杂。一些与PDT相关的凋亡途径涉及质膜死亡受体，线粒体，溶酶体和内质网（ER）。我们之前的研究发现，Photofrin主要位于线粒体中，这是Photofrin-PDT的主要靶标。在许多系统中已经证明了Bax在线粒体介导的细胞凋亡中的关键作用。为了确定Bax在Photofrin-PDT诱导的线粒体介导的细胞凋亡中的作用，我们使用了CFP / GFP-Bax质粒来监测PDT处理后Bax激活和易位的动力学。通过激光扫描共聚焦显微镜，我们发现PDT诱导了Bax从胞质转移到线粒体。但是，对于过度表达YFP-HSP70质粒的细胞，未检测到Bax易位。因此，对于Photofrin-PDT，HSP70抑制了Bax激活和易位，而不影响细胞死亡。

著录项

  • 来源
    《Biophotonics and immune responses IV》|2009年|71780D.1-71780D.8|共8页
  • 会议地点 San Jose CA(US)
  • 作者

    Feifan Zhou; Wei R.Chen; Sheng Song;

  • 作者单位

    MOE Key Laboratory of Laser Life Science Institute of Laser Life Science, South China Normal University, Guangzhou 510631, China;

    MOE Key Laboratory of Laser Life Science Institute of Laser Life Science, South China Normal University, Guangzhou 510631, China Biomedical Engineering Program, Department of Engineering and Physics, College of Mathematics and Science, University of Central Oklahoma, Edmond, Oklahoma 73034, USA;

    MOE Key Laboratory of Laser Life Science Institute of Laser Life Science, South China Normal University, Guangzhou 510631, China;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    apoptosis; photodynamic therapy (PDT); photofrin; bax; HSP70;

    机译：细胞凋亡光动力疗法（PDT）；光敏蛋白巴克斯HSP70;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号