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In vitro therapeutic effect of PDT combined with VEGF-A gene therapy

机译：PDT联合VEGF-A基因治疗的体外治疗作用

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摘要

Vascular endothelial growth factor A (VEGF-A), commonly known as VEGF, is one of the primary factors that affect tumor angiogenesis. It was found to be expressed in cancer cell lines including oral squamous cell carcinoma. Photodynamic therapy (PDT) is a novel therapeutic modality to treat cancer by using a photosensitizer which is activated by a light source to produce reactive oxygen species and mediates oxygen-independent hypoxic conditions to tumor. Another emerging treatment to cure cancer is the use of interference RNA (e.g. siRNA) to silence a specific mRNA sequence. VEGF-A was found to be expressed in oral squamous cell carcinoma and overexpressed after 24 hour post-PDT by Western blot analysis. Cell viability was found to decrease at 25 nM of transfected VEGF-A siRNA. In vitro combined therapy of PDT and VEGF-A siRNA showed better response as compared with PDT and gene therapy alone. The results suggest that PDT combined with targeted gene therapy has a potential mean to achieve better therapeutic outcome.
机译：血管内皮生长因子A（VEGF-A），通常称为VEGF，是影响肿瘤血管生成的主要因素之一。发现其在包括口腔鳞状细胞癌在内的癌细胞系中表达。光动力疗法（PDT）是一种新型的治疗方法，通过使用光敏剂来治疗癌症，该光敏剂被光源激活以产生活性氧并介导与氧无关的低氧状况。治疗癌症的另一种新兴疗法是使用干扰RNA（例如siRNA）使特定的mRNA序列沉默。通过蛋白质印迹分析，发现PDT后24小时，VEGF-A在口腔鳞状细胞癌中表达，并过表达。发现在25nM转染的VEGF-A siRNA细胞活力降低。与单独的PDT和基因治疗相比，PDT和VEGF-A siRNA的体外联合治疗显示出更好的反应。结果表明，PDT与靶向基因治疗相结合具有实现更好治疗效果的潜在手段。

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  • 来源
    《Biophotonics and immune responses IX》|2014年|894405.1-894405.6|共6页
  • 会议地点 San Francisco CA(US)
  • 作者

    Rumwald Leo G. Lecaros; Leaf Huang; Yih-Chih Hsu;

  • 作者单位

    Program of Bioscience Technology, Chung Yuan Christian University, Taoyuan,Taiwan, 32023, ROC;

    Department of Bioscience Technology, Chung Yuan Christian University, Taoyuan, Taiwan, 32023, ROC,Center of Biomedical Technology, Chung Yuan Christian University, Taoyuan, Taiwan,Division of Molecular Pharmaceutics, Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, United States;

    Department of Bioscience Technology, Chung Yuan Christian University, Taoyuan, Taiwan, 32023, ROC,Center of Biomedical Technology, Chung Yuan Christian University, Taoyuan, Taiwan,Center for Nanotechnology, Chung Yuan Christian University, Taoyuan, Taiwan, 32023, ROC;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Vascular endothelial growth factor A (VEGF-A); photodynamic therapy; siRNA; oral squamous cell carcinoma;

    机译：血管内皮生长因子A（VEGF-A）；光动力疗法； siRNA;口腔鳞状细胞癌;

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