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Anti-tumor Response Induced by Immunologically Modified Carbon Nanotubes and Laser Irradiation using Rat Mammary Tumor Model

机译:免疫修饰的碳纳米管和大鼠乳腺肿瘤模型激光辐照诱导的抗肿瘤反应。

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摘要

Laser immunotherapy (LIT) is being developed as a treatment modality for metastatic cancer which can destroy primary tumors and induce effective systemic anti-tumor responses by using a targeted treatment approach in conjunction with the use of a novel immunoadjuvant, glycated chitosan (GC). In this study, Non-invasive Laser Immunotherapy (NLIT) was used as the primary treatment mode. We incorporated single-walled carbon nanotubes (SWNTs) into the treatment regimen to boost the tumor-killing effect of LIT. SWNTs and GC were conjugated to create a completely novel, immunologically modified carbon nanotube (SWNT-GC). To determine the efficacy of different laser irradiation durations, 5 minutes or 10 minutes, a series of experiments were performed. Rats were inoculated with DMBA-4 cancer cells, a highly aggressive metastatic cancer cell line. Half of the treatment group of rats receiving laser irradiation for 10 minutes survived without primary or metastatic tumors. The treatment group of rats receiving laser irradiation for 5 minutes had no survivors. Thus, Laser+SWNT-GC treatment with 10 minutes of laser irradiation proved to be effective at reducing tumor size and inducing long-term anti-tumor immunity.
机译:激光免疫疗法(LIT)正在开发为一种转移性癌症的治疗方法,该方法可通过靶向治疗方法与新型免疫佐剂,糖化壳聚糖(GC)结合使用来破坏原发性肿瘤并诱导有效的全身性抗肿瘤反应。在这项研究中,非侵入性激光免疫疗法(NLIT)被用作主要治疗模式。我们将单壁碳纳米管(SWNTs)纳入治疗方案,以增强LIT的肿瘤杀伤作用。将SWNT和GC结合在一起以创建一个全新的,经过免疫学修饰的碳纳米管(SWNT-GC)。为了确定5分钟或10分钟的不同激光照射持续时间的功效,进行了一系列实验。用高侵袭性转移癌细胞系DMBA-4癌细胞接种大鼠。接受激光照射10分钟的大鼠治疗组中有一半存活下来,没有原发性或转移性肿瘤。接受激光照射5分钟的大鼠的治疗组没有幸存者。因此,用激光照射10分钟进行Laser + SWNT-GC处理被证明在减小肿瘤尺寸和诱导长期抗肿瘤免疫方面是有效的。

著录项

  • 来源
    《Biophotonics and immune responses X》|2015年|932415.1-932415.6|共6页
  • 会议地点 San Francisco CA(US)
  • 作者单位

    Biophotonics Research Laboratory, Center of Interdisciplinary Biomedical Education and Research, University of Central Oklahoma, Edmond Oklahoma, USA;

    Biophotonics Research Laboratory, Center of Interdisciplinary Biomedical Education and Research, University of Central Oklahoma, Edmond Oklahoma, USA;

    Biophotonics Research Laboratory, Center of Interdisciplinary Biomedical Education and Research, University of Central Oklahoma, Edmond Oklahoma, USA;

    Biophotonics Research Laboratory, Center of Interdisciplinary Biomedical Education and Research, University of Central Oklahoma, Edmond Oklahoma, USA;

    Center for Bioengineering and School of Electrical and Computer Engineering, University of Oklahoma, Norman, OK 73019, USA;

    University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA;

    University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA;

    SouthWest NanoTechnologies, Norman Oklahoma, USA;

    Biophotonics Research Laboratory, Center of Interdisciplinary Biomedical Education and Research, University of Central Oklahoma, Edmond Oklahoma, USA;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    anti-tumor response; laser immunotherapy (LIT); single-walled carbon nanotubes (SWNTs); glycated chitosan (GC);

    机译:抗肿瘤反应激光免疫疗法(LIT);单壁碳纳米管(SWNT);糖化壳聚糖(GC);

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