Vesicle adhesion depicted at nanoscale with Normalized Total Internal Reflection Fluorescence Microscopy

机译：归一化全内反射荧光显微镜在纳米尺度上描绘的囊泡粘附。

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We propose a novel fluorescence microscopy technique to study the adhesion of Giant Unilamellar Vesicles. Their adhesion is assessed through the normalization of Total Internal Reflection Fluorescence (TIRF) images by epi-fluorescence ones. This can be achieved by using a motorized rotatable mirror to switch from epi-fluorescence to TIRF. This method allows us to study the vesicle adhesion from ultra-weak to strong membrane surface interactions and to measure the absolute distance between the vesicles and various chemically functionalized glass substrates at the nanoscale.

机译：我们提出了一种新颖的荧光显微镜技术，以研究巨型单层囊泡的粘附。通过用落射荧光的图像对全内反射荧光（TIRF）图像进行归一化来评估其附着力。这可以通过使用电动旋转镜将落射荧光转换为TIRF来实现。这种方法使我们能够研究囊泡从超弱到强烈的膜表面相互作用的粘附力，并能以纳米级测量囊泡与各种化学功能化玻璃基质之间的绝对距离。

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来源
《Biophysics, Biology, and Biophotonics: the Crossroads》|2016年|97190I.1-97190I.6|共6页
会议地点 San Francisco CA(US)
作者
Marcelina Cardoso Dos Santos; Cyrille Vezy; Rodolphe Jaffiol;
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作者单位

Laboratoire de Nanotechnologie et d'Instrumentation Optique, Institut Charles Delaunay, UMR CNRS 6281, Universite de Technologie de Troyes, France;

Laboratoire de Nanotechnologie et d'Instrumentation Optique, Institut Charles Delaunay, UMR CNRS 6281, Universite de Technologie de Troyes, France;

Laboratoire de Nanotechnologie et d'Instrumentation Optique, Institut Charles Delaunay, UMR CNRS 6281, Universite de Technologie de Troyes, France;

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正文语种 eng
中图分类
关键词
Total Internal Fluorescence microscopy (TIRFM); nanoscale fluorescence imaging; Giant Unilamellar Vesicle (GUV); vesicle adhesion; surface functionalization; fluorescence microscopy;

机译：全内荧光显微镜（TIRFM）;纳米级荧光成像；巨型单层囊泡（GUV）;囊泡黏附；表面功能化荧光显微镜;

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专利

1. Nanoscale characterization of vesicle adhesion by normalized total internal reflection fluorescence microscopy [J] . Dos Santos Marcelina Cardoso, Vezy Cyrille, Jaffiol Rodolphe Biochimica et biophysica acta. Biomembranes . 2016,第6期

机译：归一化全内反射荧光显微镜技术对囊泡黏附的纳米表征
2. Signal analysis of total internal reflection fluorescent speckle microscopy (TIR-FSM) and wide-field epi-fluorescence FSM of the actin cytoskeleton and focal adhesions in living cells. [J] . Adams MC, Matov A, Yarar D, Journal of Microscopy . 2004,第Pta2期

机译：全内反射荧光斑点显微镜（TIR-FSM）和肌动蛋白细胞骨架的宽视野落射荧光FSM信号分析以及活细胞中的粘着斑。
3. Real-time imaging of synaptic vesicle exocytosis by total internal reflection fluorescence (TIRF) microscopy [J] . Mitsuharu Midorikawa Neuroscience Research: The Official Journal of the Japan Neuroscience Society . 2018,第期

机译：全内反射荧光（TIRF）显微镜突触囊泡外尿作用的实时成像
4. Vesicle adhesion depicted at nanoscale with Normalized Total Internal Reflection Fluorescence Microscopy [C] . Marcelina Cardoso Dos Santos, Cyrille Vezy, Rodolphe Jaffiol Conference on Biophysics, Biology, and Biophotonics . 2016

机译：纳米尺度的囊泡粘附性，具有归一化的全内反射荧光显微镜
5. Total internal reflection holographic microscopy (TIRHM) for quantitative phase characterization of cell-substrate adhesion. [D] . Ash, William Mason, III. 2010

机译：全内反射全息显微镜（TIRHM）用于定量量化细胞-基质粘附的相位。
6. Two forms of single-vesicle astrocyte exocytosis imaged with total internal reflection fluorescence microscopy [O] . David N. Bowser, Baljit S. Khakh 2007

机译：全内反射荧光显微镜成像的两种形式的单囊星形胶质细胞胞吐
7. Nanoscale contact line visualization based on total internal reflection fluorescence microscopy [O] . Franken, M.J.Z., Poelma, C., Westerweel, J. 2013

机译：基于全内反射荧光显微镜的纳米级接触线可视化

Vesicle adhesion depicted at nanoscale with Normalized Total Internal Reflection Fluorescence Microscopy

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