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Quantum dot-fluorescent protein pair as ratiometric pH sensor

机译：量子点荧光蛋白对作为比例式pH传感器

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摘要

Fluorescence resonance energy transfer (FRET) between a quantum dot (QD) and the pH-sensitive fluorescent protein mOrange has been used to develop a fluorescent pH-indicator that is bright and photostable enough for applications in fluorescence imaging, including the tracking of molecules through endocytic pathways. As the molar extinction coefficient of mOrange increases with pH, the ratio of the mOrange emission to the QD emission (F_A/F_D) increases sharply, producing greater than 10-fold increases in the F_a/F_d ratio between pH 4.5 and 7.5. This probe has been thoroughly characterized and it intracellular imaging potential explored.
机译：量子点（QD）与pH敏感的荧光蛋白mOrange之间的荧光共振能量转移（FRET）已用于开发荧光pH指示剂，该指示剂足够明亮且耐光，适用于荧光成像，包括通过分子跟踪内吞途径。随着mOrange的摩尔消光系数随pH的增加，mOrange发射与QD发射的比率（F_A / F_D）急剧增加，在pH 4.5和7.5之间产生的F_a / F_d比增加超过10倍。该探针已被彻底表征，并探索了其细胞内成像潜力。

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  • 来源
  • 会议地点 San Francisco CA(US)
  • 作者

    Allison M. Dennis; Gang Bao;

  • 作者单位

    Department of Biomedical Engineering at Georgia Institute of Technology and Emory University,313 Ferst Dr., Atlanta, Georgia, USA 30332 Current address: Chemistry Division and Center for Integrated Nanotechnologies, Los Alamos National Laboratory, Los Alamos, New Mexico, USA 87545;

    Department of Biomedical Engineering at Georgia Institute of Technology and Emory University,313 Ferst Dr., Atlanta, Georgia, USA 30332;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 医用物理学;
  • 关键词

    quantum dot fluorescent protein FRET biosensor pH sensor;

    机译：量子点荧光蛋白FRET生物传感器pH传感器;

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