Quantum dot-fluorescent protein pairs as novel fluorescence resonance energy transfer probes

Dennis AM; Bao G

首页> 外文期刊>Nano letters >Quantum dot-fluorescent protein pairs as novel fluorescence resonance energy transfer probes

【24h】

Quantum dot-fluorescent protein pairs as novel fluorescence resonance energy transfer probes

机译：量子点荧光蛋白对作为新型荧光共振能量转移探针

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Fluorescence resonance energy transfer (FRET) characteristics, including the efficiency, donor-acceptor distance, and binding strength of six fluorescent protein (FP)-quantum dot (OD) pairs were quantified, demonstrating that FPs are efficient acceptors for QD donors with up to 90% quenching of QD fluorescence and that polyhistidine coordination to OD core-shell surface is a straightforward and effective means of conjugating proteins to commercially available QDs. This provides a novel approach to developing QD-based FRET probes for biomedical applications.

机译：定量分析了荧光共振能量转移（FRET）特性，包括效率，供体-受体距离和六种荧光蛋白（FP）-量子点（OD）对的结合强度，表明FP是QD供体的有效受体，最高可达QD荧光的90％猝灭以及与OD核-壳表面的多组氨酸配位是将蛋白质与市售QD缀合的直接有效的手段。这为开发用于生物医学应用的基于QD的FRET探针提供了一种新颖的方法。

著录项

来源
《Nano letters》 |2008年第5期|共7页
作者
Dennis AM; Bao G;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类特种结构材料;
关键词
SEMICONDUCTOR NANOCRYSTALS; CDSE NANOCRYSTALS; CELLS; TRACKING; FRET;

机译：半导体纳米晶体;CDSE纳米晶体;细胞;跟踪;果胶;

相似文献

外文文献
中文文献
专利

1. Quantum dot-fluorescent protein pairs as novel fluorescence resonance energy transfer probes [J] . Dennis AM, Bao G Nano letters . 2008,第5期

机译：量子点荧光蛋白对作为新型荧光共振能量转移探针
2. Time-resolved fluorescence resonance energy transfer kinase assays using physiological protein substrates: Applications of terbium-fluorescein and terbium-green fluorescent protein fluorescence resonance energy transfer pairs [J] . Riddle Steven M., Vedvik Kevin L., Hanson George T., Analytical Biochemistry: An International Journal of Analytical and Preparative Methods . 2006,第1期

机译：使用生理蛋白底物的时间分辨荧光共振能量转移激酶测定：-荧光素和ter绿荧光蛋白荧光共振能量转移对的应用
3. Time-resolved fluorescence resonance energy transfer kinase assays using physiological protein substrates: Applications of terbium-fluorescein and terbium-green fluorescent protein fluorescence resonance energy transfer pairs [J] . Riddle SM, Vedvik KL, Hanson GT, Analytical Biochemistry: An International Journal of Analytical and Preparative Methods . 2006,第1期

机译：使用生理蛋白底物的时间分辨荧光共振能量转移激酶测定：-荧光素和ter绿荧光蛋白荧光共振能量转移对的应用
4. Extracting protein folding kinetics in single-pair fluorescence resonance energy transfer experiment based on wavelet analysis [C] . Guangcun Shan, Wei Huang International Congress on High-Speed Photography and Photonics . 2007

机译：基于小波分析的单对荧光共振能量转移实验中蛋白质折叠动力学的提取
5. Quantum dot-fluorescent protein pairs as fluorescence resonance energy transfer probes [D] . Dennis, Allison Marie 2009

机译：量子点荧光蛋白对作为荧光共振能量转移探针
6. Using an Amino Acid Fluorescence Resonance Energy Transfer Pair To Probe Protein Unfolding: Application to the Villin Headpiece Subdomain and the LysM Domain [O] . Julie M. Glasscock, Yongjin Zhu, Pramit Chowdhury, -1

机译：使用氨基酸荧光共振能量转移对来探测蛋白质的折叠：在Villin Headpiece子域和LysM域中的应用。
7. Quantification of protein interaction in living cells by two-photon spectral imaging with fluorescent protein fluorescence resonance energy transfer pair devoid of acceptor bleed-through [O] . Jiho Kim, Xiaolan Li, Moon-Sik Kang, 2011

机译：用荧光蛋白荧光共振能量转移对通过双光子荧光共振能量转移对活细胞蛋白质相互作用的定量

Quantum dot-fluorescent protein pairs as novel fluorescence resonance energy transfer probes

摘要

著录项

相似文献

相关主题

期刊订阅