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首页> 外文会议>Colloidal quantum dots for biomedical applications V >Biocompatible Water Soluble Quantum Dots as New Biophotonic Tools for Hematologic Cells: Applications for Flow Cell Cytometry
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Biocompatible Water Soluble Quantum Dots as New Biophotonic Tools for Hematologic Cells: Applications for Flow Cell Cytometry

机译：生物相容的水溶性量子点作为血液细胞的新生物光子工具：流式细胞术的应用

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Quantum dots (QDs) are a promising class of fluorescent probes that can be conjugated to a variety of specific cell antibodies. For this reason, simple, cheap and reproducible routes of QDs's syntheses arc the main goal of many researches in this field. The main objective of this work was to demonstrate the ability of QDs as biolabcls for flow cell cytometry analysis. We have synthesized biocompatible water soluble CdS/Cd(OH)_2 and CdTc/CdS QDs and applied them as fluorescent labels of hematologic cells. CdTe/CdS QDs was prepared using using a simple aqueous route with mercaptoacetic acid and mercaptopropionic acid as stabilizing agents. The resulting CdTe/CdS QDs can target biological membrane proteins and can also be internalized by cells. We applied the CdTe/CdS QDs as biolabels of human lymphocytes and compared the results obtained for lymphocytes treated and non-treated with pcrmeabilizing agents for cell membranes. Permeabilized cells present higher fluorescence pattern than non permeabilized ones. We associated antibody A to the CdS/Cd(OH)_2 QDs to label type A red blood cell (RBC). In this case, the O crythrocytes were used as the negative control. The results demonstrate that QDs were successfully functionalized with antibody A. There was a specific binding of QDs-antibody A to RBC membrane antigen only for A RBCs. We have also monitored QDs-hematologic cell interaction by using fluorescence microscopy. Our method shows that QDs can be conjugated to a variety of specific cell antibodies and can become a potential, highly efficient and low cost diagnostic tool for flow cell cytometry, very compatible with the lasers and filters used in this kind of equipments.
机译：量子点（QD）是一类有前途的荧光探针，可与多种特异性细胞抗体偶联。因此，简单，便宜和可重复的量子点合成路线是该领域许多研究的主要目标。这项工作的主要目的是证明QD作为流式细胞仪分析生物标签的能力。我们已经合成了生物相容的水溶性CdS / Cd（OH）_2和CdTc / CdS量子点，并将其用作血液细胞的荧光标记。 CdTe / CdS QD的制备使用简单的水性路线，巯基乙酸和巯基丙酸作为稳定剂。生成的CdTe / CdS QD可以靶向生物膜蛋白，也可以被细胞内化。我们将CdTe / CdS QDs用作人类淋巴细胞的生物标记，并比较了用膜增生剂处理和未处理的淋巴细胞获得的结果。透化细胞呈现出比未透化细胞更高的荧光模式。我们将抗体A与CdS / Cd（OH）_2 QD相关联以标记A型红细胞（RBC）。在这种情况下，将O淋巴细胞用作阴性对照。结果表明，QDs已成功用抗体A功能化。QDs-抗体A仅针对A RBCs与RBC膜抗原具有特异性结合。我们还通过使用荧光显微镜监测了QDs-血液细胞相互作用。我们的方法表明，量子点可以与多种特异性细胞抗体偶联，并且可以成为流式细胞术的一种潜在，高效且低成本的诊断工具，与此类设备中使用的激光器和滤光片非常兼容。

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