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首页> 外文会议>Conference on Biomedical Applications of Micro-and Nanoengineering, Dec 16-18, 2002, Melbourne, Australia >A microarray platform for the creation of a matrix of site-specific transformed cells
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A microarray platform for the creation of a matrix of site-specific transformed cells

机译：用于创建位点特异性转化细胞矩阵的微阵列平台

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This paper investigates the ability to modify the surface of silicon wafers for selective cell adhesion and the efficacy of solid phase transfections on the modified surface. Silicon surfaces are first modified by plasma polymerization of allylamine (ALAPP) and subsequent grafting of a protein-resistant layer of poly(ethylene oxide) (PEO) on the plasma polymer surface. Spatially controlled excimer ablation was then used to pattern the graft-copolymer surface for selective cell adhesion. X-ray photoelectron spectroscopy and contact angle measurements confirmed the creation of 2D patterns with different surface chemistry. Cell culture experiments with HEK 293 cells showed that cell attachment is limited to the ablated areas. Furthermore, cells could be transformed with plasmid DNA containing the gene for green fluorescent protein. Therefore, the biochip platform described in this paper, has the potential to be developed into a high-density array for analyzing gene products produced from a matrix of living cells.
机译：本文研究了修饰硅晶片表面以进行选择性细胞粘附的能力，以及在修饰的表面上进行固相转染的功效。硅表面首先通过烯丙胺（ALAPP）的等离子体聚合反应进行修饰，然后通过在等离子体聚合物表面上接枝聚环氧乙烷（PEO）的耐蛋白质层进行修饰。然后使用空间控制的受激准分子消融来图案化接枝共聚物表面，以进行选择性细胞粘附。 X射线光电子能谱和接触角测量证实了具有不同表面化学性质的二维图案的产生。用HEK 293细胞进行的细胞培养实验表明，细胞附着仅限于消融区域。此外，可用含有绿色荧光蛋白基因的质粒DNA转化细胞。因此，本文所述的生物芯片平台具有开发成高密度阵列以分析由活细胞基质产生的基因产物的潜力。

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