Electrical and optical methods for the manipulation and analyses of single cells

机译：用于单细胞处理和分析的电学和光学方法

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This paper describes the use of focused electric fields and focused optical fields for the high-resolution manipulation of single cells. A focused electric field, obtained with the use of ultramicroelectrodes (tip diameter approx 5 mu m), is used to electroporate and electrofuse individual cells selectively and with high spatial resolution. A focused optical field, in the form of an optical tweezer, is used to isolate single organelles from a cell as well as to position liposomes incorporated with receptors and transporters along the cell for the high-resolution sampling and probing of the cellular microenvironment.

机译：本文介绍了聚焦电场和聚焦光场在单细胞高分辨率处理中的应用。使用超微电极（尖端直径约5微米）获得的聚焦电场用于选择性地以高空间分辨率对单个细胞进行电穿孔和电融合。以光学镊子形式的聚焦光场用于从细胞中分离单个细胞器，并沿着细胞定位掺有受体和转运蛋白的脂质体，以进行高分辨率的细胞微环境采样和探测。

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来源
《Conference on Clinical Diagnostic Systems Jan 21-22, 2001, San Jose, USA》|2001年|p.1-8|共8页
会议地点 San Jose CA(US)
作者
Daniel T. Chiu; Maximilian Davidson; Anette Stroemberg; Frida Ryttsen; Owe Qrwar;
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作者单位

Department of Chemistry, University of Washington, Seattle, WA 98195-1700;

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会议组织
原文格式 PDF
正文语种 eng
中图分类基础医学;
关键词
single cells; liposomes; electroporation; electrofusion; microdissection; microelectrodes; optical trapping;

机译：单细胞脂质体电穿孔电熔;显微解剖微电极光学陷阱;

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Electrical and optical methods for the manipulation and analyses of single cells

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