• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>Conference on Genetically Engineered and Optical Probes for Biomedical Applications II; 20040124-20040127; San Jose,CA; US >Aequorin fusion proteins as bioluminescent tracers for competitive immunoassays
【24h】

Aequorin fusion proteins as bioluminescent tracers for competitive immunoassays

机译：水母发光蛋白融合蛋白作为竞争性免疫测定的生物发光示踪剂

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The use of bio- and chemiluminescence for the development of quantitative binding assays offers undoubted advantages over other detection systems, such as spectrophotometry, fluorescence, or radioactivity. Indeed, bio- and chemiluminescence detection provides similar, or even better, sensitivity and detectability than radioisotopes, while avoiding the problems of health hazards, waste disposal, and instability associated with the use of radioisotopes. Among bioluminescent labels, the calcium-activated photoprotein aequorin, originally isolated from Aequorea victoria and today available as a recombinant product, is characterized by very high detectability, down to attomole levels. It has been used as a bioluminescent label for developing a variety of highly sensitive immunoassays, using various analyte-aequorin conjugation strategies. When the analyte is a protein or a peptide, genetic engineering techniques can be used to produce protein fusions where the analyte is in-frame fused with aequorin, thus producing homogeneous one-to-one conjugation products, available in virtually unlimited amount. Various assays were developed using this strategy: a short review of the most interesting applications is presented, as well as the cloning, purification and initial characterization of an endothelin-1-aequorin conjugate suitable for developing a competitive immunoassay for endothelin-1, a potent vasoconstrictor peptide, involved in hypertension.
机译：与其他检测系统（例如分光光度法，荧光或放射性）相比，将生物发光和化学发光用于定量结合测定的发展无疑具有优势。实际上，生物发光和化学发光检测提供了与放射性同位素相似甚至更好的灵敏度和可检测性，同时避免了健康危害，废物处置以及与使用放射性同位素相关的不稳定问题。在生物发光标记中，钙激活的光蛋白水母发光蛋白（aequorin）最初是从维多利亚水母分离而来，如今可作为重组产品使用，其特征是具有很高的可检测性，低至阿托莫尔水平。使用各种分析物-水母发光蛋白偶联策略，它已被用作生物发光标记，用于开发各种高度敏感的免疫测定。当分析物是蛋白质或肽时，可以使用基因工程技术产生蛋白质融合物，其中将分析物与水母发光蛋白框内融合，从而产生均一的一对一结合产物，其数量几乎不受限制。使用这种策略开发了各种检测方法：提供了最有趣的应用的简短评论，以及适合开发针对内皮素-1的竞争性免疫检测的内皮素-1水母发光蛋白缀合物的克隆，纯化和初步表征。血管收缩肽，参与高血压。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号