Hydrogen peroxide production in cultured RPE cells exposed to near infrared lasers

机译：暴露于近红外激光的培养RPE细胞中过氧化氢的产生

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Hydrogen peroxide (H_2O_2) in situ synthesis within cultures of retinal pigment epithelial cells exposed to near-IR (NIR) continuous wave (CW) and mode-locked lasers is investigated. Any production of H_2O_2 as a consequence of NIR laser exposure is indicative of a multiphoton absorption event, resulting in an intracellular photochemical reaction. Comparisons of mode-locked and CW laser threshold energies required for the photo-oxidative reaction provide support for an enhancement in multiphoton absorption in tissues exposed to the high peak powers inherent in mode-locked laser beams. Due to different fluorescent staining properties our results indicate different mechanisms for oxidative stress from CW and mode-locked laser exposure.

机译：研究了暴露于近红外（NIR）连续波（CW）和锁模激光的视网膜色素上皮细胞培养物中过氧化氢（H_2O_2）的原位合成。 NIR激光暴露导致的任何H_2O_2产生都表明存在多光子吸收事件，从而导致细胞内光化学反应。光氧化反应所需的锁模激光和CW激光阈值能量的比较为暴露于锁模激光束固有的高峰值功率的组织中多光子吸收的增强提供了支持。由于不同的荧光染色特性，我们的结果表明CW和锁模激光曝光产生氧化应激的机制不同。

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来源
《Conference on Laser Tissue Interaction XIII: Photochemical, Photothermal, and Photomechanical, Jan 20-23, 2002, San Jose, California》|2002年|p.150-155|共6页
会议地点 San Jose CA(US)
作者
Michael L. Denton; Debbie M. Eikum; David J. Stolarski; Gary D. Noojin; Robert J. Thomas; Randolph D. Glickman; Benjamin A. Rockwell;
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作者单位

Northrop Grumman Information Technology, San Antonio, TX, USA, 78228-1330;

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会议组织
原文格式 PDF
正文语种 eng
中图分类基础医学;
关键词
RPE cells; multi-photon; mode-locked; fluorescence microscopy;

机译：RPE细胞；多光子锁模荧光显微镜;

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2. Protective effect of pyruvate upon cultured mesothelial cells exposed to 2 mM hydrogen peroxide. [J] . Shostak A, Gotloib L, Kushnier R, Nephron . 2000,第4期

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3. The effects of kahweol, a diterpene present in coffee, on the mitochondria of the human neuroblastoma SH-SY5Y cells exposed to hydrogen peroxide [J] . Furstenau Cristina Ribas, Custodio de Souza Izabel Cristina, de Oliveira Marcos Roberto Toxicology in vitro: an international journal published in association with BIBRA . 2019,第期

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4. Hydrogen peroxide production in cultured RPE cells exposed to near infrared lasers [C] . Michael L. Denton, Debbie M. Eikum, David J. Stolarski, Society of Photo-Optical Instrumentation Engineers Conference on Laser-Tissue Interaction : Photochemical, Photothermal, and Photomechanical . 2002

机译：培养的RPE细胞过氧化氢生产暴露于红外激光器
5. Impact of alkaline hydrogen peroxide pretreatment on cell wall properties that contribute to improved enzymatic digestibility of structural carbohydrates to be utilized for biofuel production. [D] . Williams, Daniel. 2014

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6. Effect of hydrogen peroxide on prostaglandin production and cellular integrity in cultured porcine aortic endothelial cells. [O] . A R Whorton, M E Montgomery, R S Kent 1985

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7. Effect of hydrogen peroxide on prostaglandin production and cellular integrity in cultured porcine aortic endothelial cells. [O] . Whorton, A R, Montgomery, M E, Kent, R S 1985

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8. Heterogeneity in c-jun gene expression in normal and malignant cells exposed to either ionizing radiation or hydrogen peroxide [R] . Horio, M. , Collart, F. R. , Huberman, E. 1993

机译：暴露于电离辐射或过氧化氢的正常和恶性细胞中c-jun基因表达的异质性

Hydrogen peroxide production in cultured RPE cells exposed to near infrared lasers

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