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首页> 外文会议>Conference on Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems Ⅻ Jan 19-20, 22, 2002 San Jose, USA >Preclinical evaluation of 5-ALA based PDT for the treatment of canine urinary bladder cancer: fluorescence distribution and response of normal urinary bladder tissue
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Preclinical evaluation of 5-ALA based PDT for the treatment of canine urinary bladder cancer: fluorescence distribution and response of normal urinary bladder tissue

机译：基于5-ALA的PDT治疗犬膀胱癌的临床前评价：正常膀胱组织的荧光分布和反应

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摘要

Photodynamic therapy (PDT) is a potential improvement over conventional therapy for urinary bladder cancer, especially using the pro-photosensitizer 5-aminolevulinic acid (ALA), which is metabolized to its active form, protoporphyrin IX (PpIX), within both normal and neoplastic epithelial tissue. The purpose of this study was to evaluate ALA-based PDT in healthy dogs as a prelude to treating dogs with transitional cell carcinoma of the urinary bladder. Methods: Twelve dogs were given oral ALA at 0, 30, 60, or 90 mg/kg. Four hours later, the dogs were sacrificed and bladder tissue was collected and frozen for low-intensity fluorescence microscopy. Fifteen dogs were given oral ALA at 0, 30, 60, or 90 mg/kg and anesthetized four hours later. The trigone was irradiated with 635 nm laser light. All dogs were necropsied after PDT (48 h or 21 d). Results: PpIX fluorescence was confined to the urinary bladder mucosa with no detectable fluorescence in the muscularis or serosa. Histopathological lesions after PDT were limited to mild inflammation. Conclusions: Oral ALA appears to be well tolerated by healthy dogs at the dosages used. Because PpIX fluorescence was confined to the mucosa, ALA-based PDT may be a useful treatment for canine lower urinary tract neoplasia.
机译：光动力疗法（PDT）是对膀胱癌常规疗法的潜在改进，尤其是使用光敏剂5-氨基乙酰丙酸（ALA），该光敏剂在正常和赘生性肿瘤中均代谢成其活性形式原卟啉IX（PpIX）。上皮组织。这项研究的目的是评估健康犬中基于ALA的PDT，以此作为治疗患有膀胱移行细胞癌的犬的前奏。方法：12只狗以0、30、60或90 mg / kg口服ALA。四小时后，处死狗，收集膀胱组织并冷冻以进行低强度荧光显微镜检查。十五只狗以0、30、60或90 mg / kg的剂量口服口服ALA，并在四小时后麻醉。用635nm的激光照射三角骨。在PDT后48小时或21天对所有的狗进行尸检。结果：PpIX荧光局限于膀胱粘膜，在肌层或浆膜中未检测到荧光。 PDT后的组织病理学损害仅限于轻度炎症。结论：健康犬在使用的剂量下口服ALA似乎具有良好的耐受性。由于PpIX荧光仅限于粘膜，因此基于ALA的PDT可能是犬下尿路肿瘤的有用治疗方法。

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