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首页> 外文会议>Conference on Light and Optics in Biomedicine Oct 23-25, 2000, Warsaw, Poland >STUDY OF KINETIC OF ANTIGENT - ANTIBODY REACTIONS WITH SCATTERING METHOD
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STUDY OF KINETIC OF ANTIGENT - ANTIBODY REACTIONS WITH SCATTERING METHOD

机译：散射法研究抗原-抗体反应动力学。

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摘要

The immune reactions of interaction antigen-antibody represent specific effect of an antigene with an antibody, which outcome are the complex immune aggregates forming precipitate in case of a soluble antigene, or agglutinate in case of a corpuscular antigene. Immonological methods which uses in the quality of carrier protein latex's polymeric microspheresis, gained name method latex agglutination. Polymeric microspheresis have the array of advantages before biological carries, which consist in the opportunity of the variation of attributes surface and size microspheresis in the broad band of meanings with the preservation of narrow distribution particles behind measurements, the putting of functional groups, necessary for bunch with ligand on stage their synthesis, in ragidity at storage. The quantitative evaluation of parameters of a response of interaction antigen-antibody in immunology is possible by optical methods on a measurement of a modification of intensity of a light stream of a solution in an outcome of a course of a reaction. Concentration of immune complexes determine both on slacking a taking place stream of light, and on a modification of intensity of a stream of light scattering suspended particles in a solution. The process light scattering by colloidal aggregates are formed from suspension micpospheresis with adsorbed on their surface protein is described. In report the physics principle of registration immune reaction by light scattering methods is concerned. The results of the effectiveness latex's preraration created on basis of the polymeric carries is described.
机译：相互作用抗原-抗体的免疫反应代表抗原与抗体的特异作用，其结果是复杂的免疫聚集体（在可溶性抗原的情况下形成沉淀），或在微粒抗原的情况下凝集。用于载体蛋白胶乳聚合微球体质量的免疫学方法，被称为胶乳凝集法。聚合微球体在生物携带之前具有一系列优势，其中包括微球体的属性表面和尺寸在广泛的意义范围内变化的机会，其中保留了测量后狭窄的分布颗粒，官能团的放置，束缚所必需配体在合成过程中处于储存状态。免疫学中相互作用抗原-抗体的反应参数的定量评估可以通过光学方法对反应过程中溶液光流强度的改变进行测量。免疫复合物的浓度既取决于松弛的光流，也取决于溶液中悬浮颗粒的光流强度的改变。描述了由胶体聚集体产生的过程光散射，该胶体聚集体由悬浮的微球形成并吸附在其表面蛋白上形成。在报告中，涉及通过光散射方法进行配准免疫反应的物理原理。描述了基于聚合物载体产生的乳胶预浸渍效果的结果。

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