• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>Conference on Light and Optics in Biomedicine Oct 23-25, 2000, Warsaw, Poland >INTERPRETATION OF THE FLUORESCENCE IMAGE ACQUIRED FOR DNA SEQUENCING
【24h】

INTERPRETATION OF THE FLUORESCENCE IMAGE ACQUIRED FOR DNA SEQUENCING

机译：DNA测序获得的荧光图像的解释

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

This paper is devoted to problems with reliability of the fluorescence analysis in investigating the DNA chains. Relationships between thermodynamic and optical properties of a given sequence are taken to consider modeling of a virtual light-directed library of oligonucleotides. Specificity of changes in optical density caused by specific interactions between pairs of DNA bases depends on the known nearest neighbor model which has been assumed here as the background. If to assume an integrated array of the known oligonucleotides (L-mers) which all have their own stable locations, after hybridization we may obtain a fluorescence result creating a matrix of micro-images; each one corresponds to the optical answer of a given oligonucleotide included in the investigated sequence spectrum. A presented concept of interpretation of fluorescence effects depends on relation between a given level of optical density and order of the bases included in oligonucleotides involved in hybridization procedure. The factors influencing fluorescence image attributes, including signal levels and stability as well as resolution and accuracy of sensing, are discussed. The obtained results of comparative computations made on a normal and simulated abnormal composition of a given human sequence are illustrated at the case that L = 6. Basing on consequences of changes in melting temperature, thermodynamic stability and levels of fluorescence light intensity, the optical spectra are presented as images made by sites of full hybridization put on a reference thermal map.
机译：本文致力于研究DNA链的荧光分析的可靠性问题。考虑给定序列的热力学和光学性质之间的关系，以考虑对寡核苷酸的虚拟光导库进行建模。由DNA碱基对之间的特异性相互作用引起的光密度变化的特异性取决于已知的最近邻居模型，在此将其作为背景。如果假设所有已知寡核苷酸（L-mers）的整合阵列均具有其自身的稳定位置，则杂交后，我们可以获得产生微图像矩阵的荧光结果。每个对应于所研究的序列谱中包括的给定寡核苷酸的光学答案。提出的解释荧光效应的概念取决于给定水平的光密度与杂交过程中涉及的寡核苷酸中所包括的碱基的顺序之间的关系。讨论了影响荧光图像属性的因素，包括信号水平和稳定性以及传感的分辨率和准确性。在L = 6的情况下，说明了对给定人类序列的正常和模拟异常组成所做的比较计算的结果。该结果基于熔化温度，热力学稳定性和荧光强度水平，光谱变化的后果呈现为由完全杂交位点制成的图像，并置于参考热图上。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号