• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>Conference on Manipulation and Analysis of Biomolecules, Cells, and Tissues Jan 28-29, 2003 San Jose, California, USA >Using POPOP's Viscosity Dependent Lifetime as a Picosecond Resolution Standard in Near-UV Fluorescence Lifetime Imaging Microscopy
【24h】

Using POPOP's Viscosity Dependent Lifetime as a Picosecond Resolution Standard in Near-UV Fluorescence Lifetime Imaging Microscopy

机译：在近紫外荧光寿命成像显微镜中使用POPOP的粘度相关寿命作为皮秒分辨率标准

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Fluorescence lifetime imaging microscopy (FLIM) using near-UV excitation is being developed to probe endogenous fluorophores in cells and tissues. Here, we describe such a UV-FLIM system and present a useful method for measuring the fluorescence lifetime discrimination of the system using the viscosity dependent lifetime of 1,4-Bis(5-phenyloxazol-2-yl)benzene (POPOP). The time-domain UV-FLIM system employed a nitrogen laser (337.1 nm) fiber-optic coupled to an inverted microscope. Wide-field fluorescence images were obtained at controlled time delays with a 200 ps gated, intensified-CCD camera. Lifetimes were calculated from the intensity decay on a pixel-by-pixel basis. The system was capable of imaging endogenous fluorescence in living cells using UV excitation. POPOP is a nanosecond lifetime standard suitable for UV excitation (325-375 nm). Its single-exponential lifetime (1.4 ns in ethanol) is comparable to endogenous lifetime values measured in living cells. Increasing solvent viscosity via the incremental addition of glycerol produced a series of POPOP lifetime standards having a range of 1 ns. The FLIM system's ability to discriminate lifetime differences of 50 ps was demonstrated using the POPOP series. Thus. POPOP's viscosity dependent lifetime represents a useful and convenient resolution standard for UV-FLIM calibration.
机译：正在开发使用近紫外线激发的荧光寿命成像显微镜（FLIM），以探测细胞和组织中的内源性荧光团。在这里，我们描述了这种UV-FLIM系统，并提出了一种有用的方法，该方法使用1,4-双（5-苯基恶唑-2-基）苯（POPOP）的粘度依赖性寿命来测量系统的荧光寿命判别。时域UV-FLIM系统采用耦合到倒置显微镜的氮激光（337.1 nm）光纤。使用200 ps的门控增强CCD相机在受控的时间延迟下获得了宽视场荧光图像。寿命是根据每个像素的强度衰减计算得出的。该系统能够使用紫外线激发成像活细胞中的内源性荧光。 POPOP是适用于紫外线激发（325-375 nm）的纳秒寿命标准。它的单指数寿命（在乙醇中为1.4 ns）与活细胞中测得的内源寿命值相当。通过增量添加甘油来增加溶剂粘度，产生了一系列POPOP寿命标准，范围为1 ns。使用POPOP系列证明了FLIM系统能够区分50 ps的寿命差异。从而。 POPOP的粘度依赖性寿命代表了UV-FLIM校准的有用且方便的分辨率标准。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号