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首页> 外文期刊>Optics Letters >Fluorescence lifetime system for microscopy and multiwell plate imaging with a blue picosecond diode laser
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Fluorescence lifetime system for microscopy and multiwell plate imaging with a blue picosecond diode laser

机译：蓝皮秒二极管激光器用于显微镜和多孔板成像的荧光寿命系统

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摘要

We report a wide-field fluorescence lifetime imaging (FLIM) system that uses a blue picosecond pulsed diode laser as the excitation source. This represents a significant miniaturization and simplification compared with other time-domain FLIM instruments that should accelerate the development of clinical and real-world applications of FLIM. We have demonstrated this instrument in two configurations: a macroimaging setup applied to multiwell plate assays of chemically and biologically interesting fluorophores and a microscope system that has been applied to imaging of tissue sections. The importance of the adjustable repetition rate of this laser source is discussed with respect to noise reduction and precision in the lifetime determination, illustrating a further significant advantage over conventional mode-locked solid-state lasers.
机译：我们报告了使用蓝色皮秒脉冲二极管激光器作为激发源的宽视场荧光寿命成像（FLIM）系统。与其他时域FLIM仪器相比，这代表了显着的小型化和简化，这将加速FLIM的临床和实际应用的发展。我们已经以两种配置展示了该仪器：用于化学和生物学意义上的荧光团的多孔板测定的宏观成像设置，以及已用于组织切片成像的显微镜系统。关于减少噪声和确定寿命的精度方面，讨论了该激光源的可调重复率的重要性，从而说明了与传统锁模固态激光器相比的另一重要优势。

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