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首页> 外文会议>Conference on Multiphoton Microscopy in the Biomedical Sciences IV; 20040125-20040127; San Jose,CA; US >Use of adaptive optics for improved multiphoton imaging
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Use of adaptive optics for improved multiphoton imaging

机译：使用自适应光学器件改善多光子成像

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摘要

We report on the integration of active optical elements in a multiphoton microscope to improve the resolution and overall image quality when imaging deeply into biological samples. Optical models were generated of sample systems and these have been compared with the performance of the complete imaging system. The active elements used were commercially available flexible membrane mirrors controlled by custom, home written, software. Significant improvements in image quality have been demonstrated using a range of optimisation routines based on the analysis of the images produced by the system, rather than with a wavefront sensor. A three-fold increase in the resolution into the sample was achieved.
机译：我们报道了在多光子显微镜中集成有源光学元件以提高成像深度并深入生物样本时的分辨率和整体图像质量。生成了样本系统的光学模型，并将其与完整成像系统的性能进行了比较。所使用的活性元素是由定制的，家用书面软件控制的可商购的柔性膜镜。基于对系统产生的图像的分析，而不是使用波前传感器，使用一系列优化例程已证明了图像质量的显着改善。样品的分离度提高了三倍。

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