Second Harmonic Imaging of Plant Polysaccharides

机译：植物多糖的二次谐波成像

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The application of second harmonic generation (SHG) microscopy to plant materials has been neglected hitherto even though it would seem to have promise for identification and characterisation of biologically and commercially important plant polysaccharides. We have found that imaging of cellulose requires rather high laser powers which are above optimal values for live cell imaging. Starch, however is easily imaged by the technique at laser fluences compatible with extended cell viability. This also has useful applications in imaging plant-derived starchy food products. Lignin in plant cell walls shows a strong 3-photon excited fluorescence which may be enhanced by resonance effects.

机译：迄今为止，尽管将二次谐波产生（SHG）显微镜应用于植物材料似乎仍被认为具有生物学和商业上重要的植物多糖的鉴定和表征的前景，但至今仍被忽略。我们已经发现，纤维素的成像需要相当高的激光功率，其高于活细胞成像的最佳值。然而，淀粉很容易通过该技术在与扩展细胞活力兼容的激光注量下成像。这在将植物来源的淀粉状食品成像中也具有有用的应用。植物细胞壁中的木质素显示出强烈的三光子激发荧光，可通过共振效应增强。

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来源
《Conference on Multiphoton Microscopy in the Biomedical Sciences IV; 20040125-20040127; San Jose,CA; US》|2004年|P.335-342|共8页
会议地点 San Jose CA(US)
作者
Guy Cox; Jose Feijo;
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作者单位

Electron Microscope Unit F09, University of Sydney, NSW 2006 Australia;

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会议组织
原文格式 PDF
正文语种 eng
中图分类光、激光生物医学;
关键词
second harmonic generation; polysaccharides; cellulose; starch 3-photon fluorescence; food microscopy;

机译：二次谐波多糖纤维素淀粉3-光子荧光食品显微镜;

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专利

1. Second-harmonic imaging of plant polysaccharides [J] . Guy Cox, Nuno Moreno, Jose Feijo Journal of biomedical optics . 2005,第2期

机译：植物多糖的二次谐波成像
2. New perfusion imaging of tissue transplants with Contrast Harmonic Ultrasound Imaging (CHI) and Magnetic Resonance Imaging (MRI) in comparison with laser-induced Indocyanine Green (ICG) luorescence angiography [J] . E.M. Jung, L. Prantl, A.G. Schreyer Clinical hemorheology and microcirculation . 2009,第1a2期

机译：与激光诱导的吲哚菁绿（ICG）发光血管造影相比，采用对比谐波超声成像（CHI）和磁共振成像（MRI）对组织移植物进行新的灌注成像
3. New perfusion imaging of tissue transplants with Contrast Harmonic Ultrasound Imaging (CHI) and Magnetic Resonance Imaging (MRI) in comparison with laser-induced Indocyanine Green (ICG) fluorescence angiography [J] . E.M. Jung, L. Prantl, A.G. Schreyer, Clinical Hemorheology and Microcirculation . 2009,第1a2期

机译：与激光诱导的吲哚菁绿（ICG）荧光血管造影相比，使用对比谐波超声成像（CHI）和磁共振成像（MRI）对组织移植物进行新的灌注成像
4. Second Harmonic Imaging of Plant Polysaccharides [C] . Guy Cox, Jose Feijo, SPIE-The International Society for Optical Engineering, Conference on multiphoton microscopy in the biomedical sciences . 2004

机译：植物多糖的二次谐波成像
5. Texture analysis applied to second harmonic generation image data for disease classification and development of a multi-view second harmonic generation imaging platform. [D] . Wen, Lianggong. 2016

机译：纹理分析应用于二次谐波生成图像数据，用于疾病分类和多视图二次谐波生成成像平台的开发。
6. Value of tissue harmonic imaging (THI) and contrast harmonic imaging (CHI) in detection and characterisation of breast tumours [O] . D.- A. Clevert, E. M. Jung, K.-P. Jungius, -1

机译：组织谐波成像（THI）和对比谐波成像（CHI）在乳腺肿瘤的检测和表征中的价值
7. Head-to-Head Comparison of Fundamental, Tissue Harmonic and Contrast Harmonic Imaging With or Without an Air-Filled Contrast Agent, Levovist, for Endocardial Border Delineation in Patients With Poor Quality Images. [O] . Hidetoshi Yoshitani, Masaaki Takeuchi, Makoto Hirose, 2002

机译：基本，组织谐波和对比谐波成像的头部对比与或没有充气造影剂，左侧血统划分的较差质量差的患者的心内膜边界描绘。

Second Harmonic Imaging of Plant Polysaccharides

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