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首页> 外文会议>Conference on Novel Optical Instrumentation for Biomedical Applications; Jun 24-25, 2003; Munich, Germany >Towards Fourier Interferometry Fluorescence Excitation/Emission Imaging of Malignant Cells combined with Photoacoustic Microscopy
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Towards Fourier Interferometry Fluorescence Excitation/Emission Imaging of Malignant Cells combined with Photoacoustic Microscopy

机译：走向傅里叶干涉法结合光声显微镜对恶性细胞进行荧光激发/发射成像。

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Dual excitation fluorescence imaging has been used as a first step towards multi-wavelength excitation/emission fluorescence spectral imaging. Target cells are transformed keratinocytes, and osteosarcoma, human breast and colon cancer cells. Mitochondrial membrane potential probes, e.g. TMRM (tetramethylrhodamine methyl ester), Mitotracker Green (Molecular Probes, Inc., Eugene OR, USA) and a recently synthesized mitochondrial oxygen probe, [PRE, (1''-pyrene butyl)-2-rhodamine ester] allow dual excitation in the UV plus in the blue-green spectral regions. Also, using the natural endogenous probe NAD(P)H, preliminary results indicate mitochondrial responses to metabolic challenges (e.g. glucose addition), plus changes in mitochonrial distribution and morphology. In terms of application to biomedicine (for diagnostics, prognostics and drug trials) three parameters have been selected in addition to the natural probe NAD(P)H, i.e. vital fluorescence probing of mitochondria, lysosomes and Golgi apparatus. It is hoped that such a multiparameter approach will allow malignant cell characterization and grading. A new area being introduced is the use of similar methodology for biotechnological applications such as the study of the hydrogen-producing alga Chlamydomonas Reinhardtii, and possible agricultural applications, such as Saccharomyces yeast for oenology. Complementation by Photoacoustic Microscopy is also contemplated, to study the internal conversion component which follows the excitation by photons.
机译：双重激发荧光成像已被用作迈向多波长激发/发射荧光光谱成像的第一步。靶细胞是转化的角质形成细胞，以及骨肉瘤，人乳腺癌和结肠癌细胞。线粒体膜电位探针，例如TMRM（四甲基若丹明甲酯），Mitotracker Green（分子探针公司，美国尤金）和最近合成的线粒体氧探针[PRE，（1''-py丁基）-2-若丹明酯]可在蓝绿色光谱区域中的UV加。此外，使用天然内源探针NAD（P）H，初步结果表明线粒体对代谢挑战（例如添加葡萄糖）的反应以及线粒体分布和形态的变化。就应用于生物医学（用于诊断，预后和药物试验）而言，除了天然探针NAD（P）H外，还选择了三个参数，即线粒体，溶酶体和高尔基体的重要荧光探测。希望这样的多参数方法将允许恶性细胞表征和分级。引入的一个新领域是在生物技术应用中使用类似的方法，例如研究产氢藻类莱茵衣藻（Chlamydomonas Reinhardtii），以及可能的农业应用，例如酿酒酵母。还可以考虑通过光声显微镜进行补充，以研究在光子激发之后的内部转换成分。

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