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首页> 外文会议>Conference on Optical Diagnostics of Living Cells Ⅴ, Jan 23-25, 2002, San Jose, USA >Measurement of adhesive forces between bacteria and protein-coated surfaces using optical tweezers
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Measurement of adhesive forces between bacteria and protein-coated surfaces using optical tweezers

机译：使用光学镊子测量细菌与蛋白涂层表面之间的粘附力

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Bacterial adhesion is a primary cause of failure in implanted medical devices. Bacteria commonly found in device-related infections, such as S. aureus, have multiple cell surface adhesins which mediate specific adhesion to molecules found in extracellular matrix and blood plasma. Adhesins recognizing fibrinogen, fibronectin, collagen, and elastin molecules have been isolated in S. aureus. We have used optical tweezers to measure the adhesive force between a single bacterium and a protein-coated surface. Various concentrations of fibronectin, fibrinogen, or whole plasma were immobilized onto 10-?m diameter polystyrene microspheres. We optically trapped a bacterium with a titanium-sapphire laser tuned to 830 nm and contacted the cell with a coated bead. We determined the minimum force necessary to separate the cell and bead. For beads coated with fibronectin and fibrinogen, detachment force values occurred as approximate integer multiples of an estimated single bond detachment force. With plasma-coated beads, only cells lacking the fibrinogen adhesin could be detached; therefore, we believe that either this adhesin is prevalent on wild-type cells, or it is preferentially adsorbed onto the beads. Additionally, the whole plasma detachment forces appeared random; therefore, we believe that many adhesins participate in bonding to plasma.
机译：细菌粘附是植入式医疗设备故障的主要原因。通常在与设备有关的感染（如金黄色葡萄球菌）中发现的细菌具有多种细胞表面粘附素，它们介导对细胞外基质和血浆中发现的分子的特异性粘附。在金黄色葡萄球菌中已分离出识别纤维蛋白原，纤连蛋白，胶原蛋白和弹性蛋白分子的粘附素。我们已经使用光镊来测量单个细菌和蛋白涂层表面之间的粘附力。将各种浓度的纤连蛋白，纤维蛋白原或整个血浆固定在直径10微米的聚苯乙烯微球上。我们用调谐至830 nm的钛蓝宝石激光将细菌捕获，并使细胞与包被的珠子接触。我们确定了分离电池和珠子所需的最小力。对于涂覆有纤连蛋白和纤维蛋白原的珠子，剥离力值是估计的单键剥离力的近似整数倍。用血浆包被的珠子，只有缺乏纤维蛋白原粘附素的细胞才能被分离。因此，我们认为这种粘附素普遍存在于野生型细胞上，或者优先吸附在微珠上。另外，整个血浆分离力似乎是随机的。因此，我们认为许多粘附素参与与血浆的键合。

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