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首页> 外文会议>Conference on Optical Diagnostics of Living Cells Ⅴ, Jan 23-25, 2002, San Jose, USA >Imaging Microscopic Fluid Viscosity and Velocity Fields using Confocal Scanning Optical Tweezers
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Imaging Microscopic Fluid Viscosity and Velocity Fields using Confocal Scanning Optical Tweezers

机译：使用共聚焦扫描光学镊子成像微观流体粘度和速度场

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摘要

Confocal microscopy and optical tweezers were combined to develop a minimally invasive instrument capable of making hydrodynamic measurements more rapidly than is possible with other devices. This result leads to the possibility of making scanning images of the viscosity distribution of materials around biopolymer producing cells. An image of the viscosity distribution around a pullulan producing cell of Aureobasidium pullulans is shown as an example. We present results from experiments supporting a linearized model for the motion of a trapped bead in an oscillating harmonic potential. Fluid velocity measurements are tested by comparing to an independent video based measurement. We apply the technique to obtain a 2-D map of the flow past a microscopic wedge and compare to a theoretical solution for the stream lines assuming potential flow. Since the velocity is measured simultaneously with the trap relaxation time, it requires practically no calibration and is independent of the trap stiffness and the particle size.
机译：共焦显微镜和光学镊子相结合，开发出一种微创仪器，能够比其他设备更快地进行水动力测量。该结果导致可能对生物聚合物产生细胞周围的材料的粘度分布进行扫描图像。作为示例，示出了金黄色葡萄球菌的支链淀粉产生细胞周围的粘度分布的图像。我们目前的实验结果支持在振动谐波势中捕获磁珠运动的线性化模型。通过与基于独立视频的测量结果进行比较来测试流体速度测量结果。我们应用该技术获得流过微观楔形物的二维流图，并与假定潜在流量的流线理论解进行比较。由于速度是与捕集阱弛豫时间同时测量的，因此实际上不需要校准，并且不受捕集阱刚度和粒径的影响。

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