首页> 外文会议>Conference on Optical Technologies in Biophysics and Medicine IV; Oct 1-4, 2002; Saratov, Russia >Spectroscopic fluorescence measurements of lamb and human heart tissue in vitro
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Spectroscopic fluorescence measurements of lamb and human heart tissue in vitro

机译:羊和人心脏组织的光谱荧光测量

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Laser-induced fluorescence spectra were obtained during the exposure of lamb heart (n=20) tissue to Argon-ion radiation (457.9nm). Fluorescence spectra from different heart compartments (the left and right atria and ventricles, the myocardium, the epicardium, and the aorta) were recorded. Simple algebraic algorithms based on the spectral intensity variation were constructed in order to detect spectral features and characterize the different cardiac compartments. Additionally, it was investigated whether each chamber exhibited constant spectral response. After the end of each experiment the lamb hearts were stored in formalin (10%). The samples were irradiated again after forty eight (48) hours in order to investigate the spectral differences that appear due to formalin conservation. Similar fluorescence measurements were taken from a limited number of human heart tissues (n=2) ex vivo.
机译:在羔羊心脏(n = 20)组织暴露于氩离子辐射(457.9nm)的过程中获得了激光诱导的荧光光谱。记录了来自不同心脏区室(左,右心房和心室,心肌,心外膜和主动脉)的荧光光谱。构建了基于谱强度变化的简单代数算法,以检测谱特征并表征不同的心脏腔室。另外,研究了每个室是否表现出恒定的光谱响应。在每个实验结束后,将羊羔心脏保存在福尔马林中(10%)。四十八(48)小时后再次照射样品,以调查由于福尔马林保存而出现的光谱差异。从有限数量的人体心脏组织(n = 2)进行了类似的荧光测量。

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