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首页> 外文会议>Conference on Optical Tomography and Spectroscopy of Tissue V Jan 26-29, 2003 San Jose, California, USA >Three-dimensional Optical Tomographic Brain Imaging during Hypercapnia in Rodents
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Three-dimensional Optical Tomographic Brain Imaging during Hypercapnia in Rodents

机译：啮齿动物高碳酸血症期间的三维光学断层扫描脑成像

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Noninvasive examination of the hemodynamics of brain tissue is of general interest in many areas of medicine and physiology. To date, optical brain studies generate topographic maps, where signals obtained form within the brain are projected onto two-dimensional surface maps. Recently, our group has presented the first three-dimensional, volumetric reconstruction of hemodynamic changes during a Valsalva maneuver in the human forehead. To further validate our three-dimensional diffusion optical tomographic reconstruction algorithm we have turned to experimental studies involving small animals. Here we report on hypercapnia studies performed with 3-month old Sprague-Dawley rats. After anesthetizing the animal a tracheotomy was performed and the rat was artificially respirated. The head shaven and secured in a stereotaxic frame an optical probe was positioned between the bregma and lambda skull landmarks. A baseline measurement was recorded and then the inspired gas content was altered. The experimental studies verified the ability of our code to three-dimensionally visualize a global hemodynamic phenomenon in the rat head in response to perturbations in the inspired CO_2 concentrations. Specifically, we incrementally increased the concentration of inspiratory CO_2 (hypercapnia) and visualized the resulting hemodynamic change. We observed a global increase in blood volume and oxygenation, which was consistent with the known physiologic response to hypercapnia. A second set of experiments were designed to determine the sensitivity of the reconstruction to inaccurate probe positioning versus assumed model optrode-position mismatch. We determined that shifts on the order of 1/10 the maximum optrode separation significantly influence the reconstruction and may falsely produce lateralized effects.
机译：在医学和生理学的许多领域中，对脑组织的血液动力学的无创性检查普遍感兴趣。迄今为止，光学脑研究生成了地形图，其中从大脑内部获得的信号被投影到二维表面图上。最近，我们小组提出了人类额头上的Valsalva动作期间血液动力学变化的第一个三维三维重建。为了进一步验证我们的三维扩散光学层析成像重建算法，我们转向了涉及小动物的实验研究。在这里，我们报告了对3个月大的Sprague-Dawley大鼠进行的高碳酸血症研究。麻醉动物后，进行气管切开术，并人工呼吸大鼠。剃光头并将其固定在立体定位框架中，将光学探针放置在前reg和lambda头骨界标之间。记录基线测量，然后改变吸气量。实验研究证实了我们的代码能够三维响应可视化的CO_2浓度扰动，从而在大鼠头部三维整体血流动力学现象。具体来说，我们逐渐增加了吸气CO_2（高碳酸血症）的浓度，并可视化了由此产生的血液动力学变化。我们观察到全球血容量和氧合增加，这与对高碳酸血症的已知生理反应一致。设计了第二组实验以确定相对于假定的模型对极位置不匹配的不准确探针定位的重建敏感性。我们确定，最大光极间距的1/10数量级偏移会显着影响重建，并可能错误地产生侧向效应。

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