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首页> 外文会议>Conference on Optics in Tissue Engineering and Regenerative Medicine; 20080120-21; San Jose,CA(US) >Optically characterizing vascular tissue constructs made with soluble vs. homogenized collagen
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Optically characterizing vascular tissue constructs made with soluble vs. homogenized collagen

机译：用可溶性和均质胶原制成的光学表征血管组织构建体

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摘要

The ability of optical imaging techniques such as optical coherence tomography (OCT) to non-destructively characterize tissue-engineered constructs has generated enormous interest recently. We are testing the hypothesis that OCT data can be used to characterize the cellularity of collagen-based vascular constructs made from 2 types of collagen scaffold matrix: soluble collagen and homogenized collagen. Smooth muscle cells were seeded in these 2 scaffold matrices at a seeding density of 1 × 10~6 cells/ml. The disk-shaped constructs were allowed to remodel and compact in the incubator for 96 hours. OCT imaging of the constructs occurred at 24 hour intervals. From the OCT data, the attenuation and reflectivity were evaluated by fitting the data to a theoretical model that relates the tissue optical properties (scattering coefficient and anisotropy factor) and imaging conditions to the OCT signal. The fitted optical properties were compared to the construct volume. Representative H&E histological sections of the constructs were used to assess cell proliferation. Our data showed that the optical properties of the solubilized constructs changed over time while those of the homogenized constructs did not, in agreement with the histology and compaction observations.
机译：近来，诸如光学相干断层扫描（OCT）的光学成像技术无损表征组织工程构造的能力。我们正在测试以下假设：OCT数据可用于表征由两种类型的胶原蛋白支架基质（可溶性胶原蛋白和均质胶原蛋白）制成的基于胶原蛋白的血管结构的细胞性。将平滑肌细胞以1×10〜6细胞/ ml的接种密度接种在这两种支架基质中。盘状构建体在培养箱中重塑并压实96小时。对构建体的OCT成像每隔24小时发生一次。根据OCT数据，通过将数据拟合到将组织光学特性（散射系数和各向异性因子）和成像条件与OCT信号相关联的理论模型，来评估衰减和反射率。将拟合的光学性质与构建体体积进行比较。构建体的代表性H＆E组织学切片用于评估细胞增殖。我们的数据表明，与组织学和压实观察结果一致，可溶解的构建体的光学性质会随时间而变化，而均匀化的构建体的光学性质不会随时间变化。

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