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首页> 外文会议>Conference on Optics in Tissue Engineering and Regenerative Medicine; 20080120-21; San Jose,CA(US) >Imaging stented tissue engineered blood vessel mimics
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Imaging stented tissue engineered blood vessel mimics

机译：成像支架组织工程血管模拟

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An ideal vascular stent design promotes a thin anti-thrombogenic cellular lining while avoiding restenosis. To assess the utility of their designs, stent manufactures often use destructive techniques such as scanning electron microscopy to measure the percentage of the stent covered with a cellular lining. In this study, we use a custom-built longitudinal/rotational scanning endoscope and determine the ability of optical coherence tomography (OCT) to quantify the percent cellular coverage of stented tissue engineered blood vessel mimics. Stents were deployed within twelve mimics after 14-days of development in bioreactors. OCT images were acquired within the bioreactor at several time points after the stent deployment. At 20-days post deployment, the mimics were fixed and imaged volumetrically with OCT. Matlab software was developed to automatically calculate the percent cellular coverage from the OCT images. Algorithm results were compared to similar measurements performed with bis-benzimide (BBI) fluorescence imaging and manually calculated percent coverage from three different observers of the OCT images. Progressive accumulation of cellular material on the stents could be visualized with OCT. For the volumetric images, the algorithm calculated percent cellular coverages ranging from 11 to 76%. Good agreement was found between the OCT-based measurements and the other techniques. On average, the algorithm differed less than 5% from the manual percent coverage calculations. OCT together with automated software can provide an accurate, non-destructive measurement of the percent cellular coverage of vascular stents.
机译：理想的血管支架设计可促进薄的抗血栓形成的细胞内衬，同时避免再狭窄。为了评估其设计的实用性，支架制造商经常使用破坏性技术（例如扫描电子显微镜）来测量覆盖有细胞衬里的支架的百分比。在这项研究中，我们使用定制的纵向/旋转扫描内窥镜并确定光学相干断层扫描（OCT）的能力，以量化支架组织工程化血管模拟物的细胞覆盖百分比。在生物反应器中开发14天后，在十二个模拟物中部署了支架。在支架展开后的几个时间点，在生物反应器中获取了OCT图像。部署后20天，模拟对象被固定并使用OCT进行体积成像。 Matlab软件的开发是根据OCT图像自动计算细胞覆盖百分比。将算法结果与使用双苯甲酰亚胺（BBI）荧光成像进行的类似测量进行比较，并从OCT图像的三个不同观察者手动计算覆盖率。 OCT可显示细胞材料在支架上的逐渐积累。对于体积图像，该算法计算的细胞覆盖百分比范围为11％到76％。在基于OCT的测量与其他技术之间发现了很好的一致性。平均而言，该算法与手动覆盖率百分比计算的差异小于5％。 OCT与自动化软件一起可以准确，无损地测量血管支架的细胞覆盖百分比。

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