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首页> 外文会议>Conference on Optics in Tissue Engineering and Regenerative Medicine; 20080120-21; San Jose,CA(US) >Determination of 3D optic axis orientation in cartilage by Polarization- Sensitive Optical Coherence Tomography
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Determination of 3D optic axis orientation in cartilage by Polarization- Sensitive Optical Coherence Tomography

机译：偏振敏感光学相干层析成像技术确定软骨中3D光轴的方向

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摘要

Polarization-sensitive optical coherence tomography has been used to solve fast-axis fibre orientation in three dimension space. Previously we have demonstrated that the apparent variations in polar angle orientation of collagen fibers along sagittal ridge of equine third metacarpophalangeal joint exist. A quantitative method based on multiple angles of illumination has been proposed to determine the polar angle of the collagen fibers. This method however ignored the full 3D structure by assuming that the collagen fibers long-axis lay within the plane of incidence. A new quantitative method based on the theory of light propagation in uniaxial materials is described which avoids this assumption. To test this method we have performed control experiments on a sample of equine tendon (this tissue has well defined c-axis lying along the long-axis of the tendon). Several samples of tendon were cut to achieve a planar surface inclined at -20° to the long axis. Additional 30° rotation provided non-zero azimuthal angle. The surface was then imaged using incident beam angles -40°, -20°, 0, +20°, +40° in two orthogonal planes. Values for both the polar and azimuthal angles were then derived using a numerical optimisation procedure. Results agreed qualitatively with the nominal values but suggested that the accuracy was limited by our method of determining the apparent birefringence.
机译：偏振敏感光学相干断层扫描已用于解决三维空间中的快轴光纤定向问题。先前我们已经证明，沿着马第三掌指关节的矢状脊存在胶原纤维的极角取向的明显变化。已经提出了一种基于多个照明角度的定量方法来确定胶原纤维的极角。但是，该方法通过假设胶原纤维的长轴位于入射平面内而忽略了完整的3D结构。描述了一种基于光在单轴材料中传播的理论的新定量方法，该方法避免了这种假设。为了测试此方法，我们对马肌腱样本进行了对照实验（该组织的C轴沿肌腱的长轴分布明确）。切下几个肌腱样品，以获得相对于长轴倾斜-20°的平面。额外旋转30°可提供非零方位角。然后在两个正交平面中使用入射角-40°，-20°，0，+ 20°，+ 40°对表面成像。然后使用数值优化程序导出极角和方位角的值。结果在质量上与标称值一致，但表明精度受到我们确定表观双折射的方法的限制。

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