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首页> 外文会议>Conference on Photon Processing in Microelectronics and Photonics Jan 21-24, 2002 San Jose, USA >Cell-by-Cell Construction of Living Tissue
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Cell-by-Cell Construction of Living Tissue

机译：活组织的逐细胞构建

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This paper outlines investigations into a potentially revolutionary approach to tissue engineering. Tissue is a complex three-dimensional structure that contains many different biomaterials such as cells, proteins, and extracellular matrix molecules that are ordered in a very precise way to serve specific functions. In order to replicate such complex structure, it is necessary to have a tool that could deposit all these materials in an accurate and controlled fashion. Most methods to fabricate living three-dimensional structures involve techniques to engineer biocompatible and biodegradable scaffolding, which is then seeded with living cells to form tissue. This scaffolding gives the tissue needed support, but the resulting tissue inherently has no microscopic cellular structure because cells are injected into the scaffolding where they adhere at random. We have developed a novel technique that actually engineers tissue, not scaffolding, that includes the mesoscopic cellular structure inherent in natural tissues. This approach uses a laser-based rapid prototyping system known as matrix assisted pulsed laser evaporation direct write (MAPLE DW) to construct living tissue cell-by-cell. This manuscript details our efforts to rapidly and reproducibly fabricate complex 2D and 3D tissue structures with MAPLE-DW by placing different cells and biomaterials accurately and adherently on the mesoscopic scale.
机译：本文概述了对组织工程潜在革命性方法的研究。组织是复杂的三维结构，其中包含许多不同的生物材料，例如细胞，蛋白质和细胞外基质分子，它们以非常精确的方式排列以发挥特定功能。为了复制这种复杂的结构，有必要提供一种能够以精确且可控的方式沉积所有这些材料的工具。制造活体三维结构的大多数方法都涉及工程化生物相容性和可生物降解支架的技术，然后将其与活细胞一起接种以形成组织。该支架为组织提供了所需的支撑，但是由于将细胞注射到支架中并随机粘附，因此所得组织固有地没有微观的细胞结构。我们已经开发了一种新技术，该技术实际上是在组织而不是在脚手架上进行工程设计的，其中包括天然组织固有的介观细胞结构。这种方法使用了基于激光的快速原型系统，称为基质辅助脉冲激光蒸发直接写入（MAPLE DW），可以逐细胞构建活组织。这份手稿详细介绍了我们通过MAPLE-DW快速而可重复地制造复杂的2D和3D组织结构的努力，方法是将不同的细胞和生物材料精确且粘附地置于介观尺度上。

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