Visualization of EGFR and Grb2 Interaction Using Bimolecular Fluorescence Complementation

机译：使用双分子荧光互补物观察EGFR和Grb2的相互作用

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The dimerization of epidermal growth factor receptor (EGFR) and its endocytic transport are important in regulating signal transduction. In the present study, we applied the strategy of Bimolecular Fluorescence Complementation (BiFC), EGFR homodimer and hetrodimer of EGFR and its partner Grb2 in cells were visualized. This strategy takes advantage of the ability of two nonfluorescent fragments of Venus fluorescent protein to form a fluorescent signal when fused to the amino termini of EGFR and Grb2. Since EGFR is a large protein contains more than 1800 amido acids, proper fold of the fusion protein is essential for the BiFC assay of EGFR and its partners. Our results indicate that BiFC is a suitable application for research of EGFR interaction with other proteins.

机译：表皮生长因子受体（EGFR）的二聚化及其内吞转运在调节信号转导中很重要。在本研究中，我们应用了双分子荧光互补（BiFC）策略，将EGFR及其同伴Grb2在细胞中的EGFR同型二聚体和异型二聚体可视化。当与EGFR和Grb2的氨基末端融合时，该策略利用了维纳斯荧光蛋白的两个非荧光片段形成荧光信号的能力。由于EGFR是包含1800多个酰胺基酸的大蛋白，因此融合蛋白的正确折叠对于EGFR及其伴侣的BiFC测定至关重要。我们的结果表明，BiFC是研究EGFR与其他蛋白质相互作用的合适应用。

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来源
《Conference on Small Animal Whole-Body Optical Imaging Based on Genetically Engineered Probes; 20080121-22; San Jose,CA(US)》|2008年|P.iv|共2页
会议地点 San JoseCA(US)
作者
Xiangyong Li; Jun Chu; Qingming Luo; Zhihong Zhang;
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作者单位

Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong university of Science and Technology, 430074, P.R. China;

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会议组织
原文格式 PDF
正文语种 eng
中图分类医用物理学;
关键词
bimolecular fluorescence complementation; proteins interaction; EGFR; internalization;

机译：双分子荧光互补；蛋白质相互作用； EGFR；内在化;

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1. Visualization of molecular interactions using bimolecular fluorescence complementation analysis: Characteristics of protein fragment complementation [J] . Tom K. Kerppola Chemical Society Reviews . 2009,第10期

机译：使用双分子荧光互补分析可视化分子相互作用：蛋白质片段互补的特征
2. Visualization of cofilin-actin and Ras-Raf interactions by bimolecular fluorescence complementation assays using a new pair of split Venus fragments [J] . Ohashi Kazumasa, Kiuchi Tai, Shoji Kazuyasu, BioTechniques . 2012,第1期

机译：通过使用一对新的分裂维纳斯片段的双分子荧光互补测定法可视化cofilin-actin和Ras-Raf相互作用
3. Visualization of protein interactions in living Drosophila embryos by the bimolecular fluorescence complementation assay [J] . Bruno Hudry, Séverine Viala, Yacine Graba, BMC Biology . 2011,第1期

机译：通过双分子荧光互补测定法观察果蝇活体胚胎中蛋白质的相互作用
4. Visualization of EGFR and Grb2 Interaction Using Bimolecular Fluorescence Complementation [C] . Xiangyong Li, Jun Chu, Qingming Luo, Conference on Small Animal Whole-Body Optical Imaging Based on Genetically Engineered Probes . 2008

机译：使用双分子荧光互补的EGFR和GRB2相互作用的可视化
5. Protein interactions in gametophytic self-incompatibility: Bimolecular fluorescence complementation assays in Petunia hybrida and data-mining for SLF-variant sequences in Petunia inflata. [D] . Malla, Diwa. 2013

机译：配子体自交不亲和中的蛋白质相互作用：矮牵牛中的双分子荧光互补测定法和矮牵牛中SLF变异序列的数据挖掘。
6. VISUALIZATION OF MOLECULAR INTERACTIONS USING BIMOLECULAR FLUORESCENCE COMPLEMENTATION ANALYSIS: CHARACTERISTICS OF PROTEIN FRAGMENT COMPLEMENTATION [O] . Tom K. Kerppola -1

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7. Visualization of protein interactions in living Drosophila embryos by the bimolecular fluorescence complementation assay [O] . Bruno Hudry, Séverine Viala, Yacine Graba, 2011

机译：通过双分子荧光互补测定法观察果蝇活体胚胎中的蛋白质相互作用
8. Bimolecular Complementation to Visualize Filovirus VP40-Host Complexes in Live Mammalian Cells: Toward the Identification of Budding Inhibitors [R] . Liu, Y., Lee, M. S., Olson, M. A., 2011

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Visualization of EGFR and Grb2 Interaction Using Bimolecular Fluorescence Complementation

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