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首页> 外文会议>Conference on Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing Ⅷ Jan 21-22, 2001, San Jose, USA >Optical Biopsy of Normal and Cacnerous Cervical Tissues by Native Fluorescence Spectroscopy
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Optical Biopsy of Normal and Cacnerous Cervical Tissues by Native Fluorescence Spectroscopy

机译：通过天然荧光光谱对正常和颈椎宫颈组织进行光学活检

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摘要

Native fluorescence spectra of tissues of both normal and cervical carcinoma were measured at 320 nm excitation. The spectra were normalized with respect to maximum peak intensity and average fluorescence spectra were computed for normal and cancerous cervical tissues. The average fluorescence spectrum of normal cervix is found to be significantly different from that of cancer. Both normal and cancerous cervical tissues show a distinct primary emission peak around 380 nm which may be attributed to the structural proteins, collagen and elastin. In addition, the average spectrum of normal cervix shows a less intense peak around 440 nm, which is red shifted to 460nm and more intense in the case of cancerous cervical tissues, when compared to normal. A ratio parameter I_(380)/I_(460) is introduced and a critical value of 1.9 for this ratio parameter is found to classify cervical cancerous tissues from normal with a sensitivity and specificity of 100 percent and 85.7 percent respectively. Also the slope value analysis between 420 to 440nm & 440 to 460nm of the normal and cancerous samples gives 100 percent accuracy in the classification of normal from the cancerous samples. Further, discriminant analysis together with Principal component analysis (PCA) was performed to determine the statistical significance and to confirm the above two analysis. The potentiality of the ratio parameter and slope values analysis is improved by the support of multivariate statistical analysis.
机译：在320 nm激发下测量正常和宫颈癌组织的天然荧光光谱。相对于最大峰强度对光谱进行归一化，并计算正常和癌性宫颈组织的平均荧光光谱。发现正常子宫颈的平均荧光光谱与癌症的显着不同。正常和癌性宫颈组织均在380 nm附近显示出明显的主要发射峰，这可能归因于结构蛋白，胶原蛋白和弹性蛋白。此外，正常宫颈的平均光谱在440 nm附近显示一个较小的峰，与正常宫颈相比，在宫颈癌变组织中红色移至460 nm，而在宫颈癌组织中则更强烈。引入比率参数I_（380）/ I_（460），发现该比率参数的临界值为1.9，可将宫颈癌组织与正常组织区分为敏感性和特异性，分别为100％和85.7％。同样，正常和癌性样品在420至440nm和440至460nm之间的斜率分析可对癌性样品的正常分类提供100％的准确性。此外，进行了判别分析和主成分分析（PCA）以确定统计显着性并确认上述两种分析。通过多元统计分析的支持，可以提高比率参数和斜率值分析的潜力。

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