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首页> 外文会议>Diagnostic Optical Spectroscopy in Biomedicine IV; Progress in Biomedical Optics and Imaging; vol.8 no.41; Proceedings of SPIE-The International Society for Optical Engineering; vol.6628 >Spectroscopic imaging using acousto-optic tuneable filters
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Spectroscopic imaging using acousto-optic tuneable filters

机译：使用声光可调滤镜的光谱成像

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摘要

We report on novel hyper-spectral imaging filter-modules based on acousto-optic tuneable filters (AOTF). The AOTF functions as a full-field tuneable bandpass filter which offers fast continuous or random access tuning with high filtering efficiency. Due to the diffractive nature of the device, the unfiltered zero-order and the filtered first-order images are geometrically separated. The modules developed exploit this feature to simultaneously route both the transmitted white-light image and the filtered fluorescence image to two separate cameras. Incorporation of prisms in the optical paths and careful design of the relay optics in the filter module have overcome a number of aberrations inherent to imaging through AOTFs, leading to excellent spatial resolution. A number of practical uses of this technique, both for in vivo auto-fluorescence endoscopy and in vitro fluorescence microscopy were demonstrated. We describe the operational principle and design of recently improved prototype instruments for fluorescence-based diagnostics and demonstrate their performance by presenting challenging hyper-spectral fluorescence imaging applications.
机译：我们报告基于声光可调谐滤波器（AOTF）的新型高光谱成像滤波器模块。 AOTF用作全场可调谐带通滤波器，可提供快速连续或随机访问调整，并具有很高的滤波效率。由于设备的衍射特性，未过滤的零阶图像和已过滤的一阶图像在几何上是分开的。开发的模块利用此功能将发送的白光图像和滤波后的荧光图像同时路由到两个单独的摄像机。在光路中合并棱镜以及在滤波器模块中精心设计中继光学器件，已克服了通过AOTF成像所固有的许多像差，从而带来了出色的空间分辨率。证明了该技术在体内自发荧光内窥镜检查和体外荧光显微术中的许多实际应用。我们描述了用于基于荧光的诊断的最近改进的原型仪器的操作原理和设计，并通过提出具有挑战性的高光谱荧光成像应用来证明其性能。

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