• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>Energy-based treatment of tissue and assessment VIII >External stimulation by nanosecond pulsed electric fields to enhance cellular uptake of nanoparticles
【24h】

External stimulation by nanosecond pulsed electric fields to enhance cellular uptake of nanoparticles

机译：纳秒脉冲电场的外部刺激可增强纳米颗粒的细胞吸收

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

As an increasing number of studies use gold nanoparticles (AuNPs) for potential medicinal, biosensing and therapeutic applications, the synthesis and use of readily functional, bio-compatible nanoparticles is receiving much interest. For these efforts, the particles are often taken up by the cells to allow for optimum sensing or therapeutic measures. This process typically requires incubation of the particles with the cells for an extended period. In an attempt to shorten and control this incubation, we investigated whether nanosecond pulsed electric field (nsPEF) exposure of cells will cause a controlled uptake of the particles. NsPEF are known to induce the formation of nanopores in the plasma membrane, so we hypothesized that by controlling the number, amplitude or duration of the nsPEF exposure, we could control the size of the nanopores, and thus control the particle uptake. Chinese hamster ovary (CHO-K1) cells were incubated sub-10 nm AuNPs with and without exposure to 600-ns electrical pulses. Contrary to our hypothesis, the nsPEF exposure was found to actually decrease the particle uptake in the exposed cells. This result suggests that the nsPEF exposure may be affecting the endocytotic pathway and processes due to membrane disruption.
机译：随着越来越多的研究将金纳米颗粒（AuNPs）用于潜在的医学，生物传感和治疗应用，易于使用的，具有生物相容性的纳米颗粒的合成和使用受到了广泛的关注。为了这些努力，颗粒通常被细胞吸收以允许最佳的感测或治疗措施。该过程通常需要将颗粒与细胞孵育一段较长的时间。为了缩短和控制这种温育，我们研究了细胞的纳秒脉冲电场（nsPEF）暴露是否会引起颗粒的受控摄取。已知NsPEF会诱导质膜中形成纳米孔，因此我们假设通过控制nsPEF暴露的数量，幅度或持续时间，我们可以控制纳米孔的大小，从而控制颗粒的吸收。将中国仓鼠卵巢（CHO-K1）细胞在暴露于和未暴露于600 ns电脉冲的情况下在10 nm以下AuNPs下孵育。与我们的假设相反，发现nsPEF暴露实际上减少了暴露细胞中的颗粒摄取。该结果表明nsPEF暴露可能由于膜破裂而影响内吞途径和过程。

著录项

  • 来源
  • 会议地点 San Francisco CA(US)
  • 作者

    Samantha Franklin; Hope T. Beier; Bennett L. Ibey; Kelly Nash;

  • 作者单位

    Department of Physics-University of Texas at San Antonio, 1 UTSA Circle, San Antonio TX 78249, 711th Human Performance Wing, Human Effectiveness Directorate, Bioeffects Division, Radio Frequency Bioeffects Branch, 4141 Petroleum Drive, JBSA Fort Sam Houston TX 78234;

    711th Human Performance Wing, Human Effectiveness Directorate, Bioeffects Division, Optical Radiation Bioeffects Branch, 4141 Petroleum Drive, JBSA Fort Sam Houston TX 78234;

    711th Human Performance Wing, Human Effectiveness Directorate, Bioeffects Division, Radio Frequency Bioeffects Branch, 4141 Petroleum Drive, JBSA Fort Sam Houston TX 78234;

    Department of Physics-University of Texas at San Antonio, 1 UTSA Circle, San Antonio TX 78249;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    nanosecond electrical pulses; gold nanoparticles; chitosan; cellular uptake;

    机译：纳秒电脉冲；金纳米粒子壳聚糖细胞摄取;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号