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首页> 外文会议>Conference on energy-based treatment of tissue and assessment VIII >External stimulation by nanosecond pulsed electric fields to enhance cellular uptake of nanoparticles
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External stimulation by nanosecond pulsed electric fields to enhance cellular uptake of nanoparticles

机译：纳秒脉冲电场外部刺激，增强纳米颗粒的细胞摄取

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As an increasing number of studies use gold nanoparticles (AuNPs) for potential medicinal, biosensing and therapeutic applications, the synthesis and use of readily functional, bio-compatible nanoparticles is receiving much interest. For these efforts, the particles are often taken up by the cells to allow for optimum sensing or therapeutic measures. This process typically requires incubation of the particles with the cells for an extended period. In an attempt to shorten and control this incubation, we investigated whether nanosecond pulsed electric field (nsPEF) exposure of cells will cause a controlled uptake of the particles. NsPEF are known to induce the formation of nanopores in the plasma membrane, so we hypothesized that by controlling the number, amplitude or duration of the nsPEF exposure, we could control the size of the nanopores, and thus control the particle uptake. Chinese hamster ovary (CHO-K1) cells were incubated sub-10 nm AuNPs with and without exposure to 600-ns electrical pulses. Contrary to our hypothesis, the nsPEF exposure was found to actually decrease the particle uptake in the exposed cells. This result suggests that the nsPEF exposure may be affecting the endocytotic pathway and processes due to membrane disruption.
机译：作为越来越多的研究，使用金纳米颗粒（AUNP）用于潜在的药用，生物溶剂和治疗应用，易于功能的合成和使用，生物相容的纳米颗粒是非常感兴趣的。对于这些努力，颗粒通常被细胞占用以允许最佳感测或治疗措施。该方法通常需要延长颗粒与细胞孵育。为了试图缩短和控制这种孵化，我们研究了细胞的纳秒脉冲电场（nspef）暴露是否会导致受控的颗粒摄取。已知NPEF在质膜中诱导纳米孔的形成，因此我们假设通过控制NPEF暴露的数量，幅度或持续时间，我们可以控制纳米孔的尺寸，从而控制粒子摄取。中国仓鼠卵巢（CHO-K1）细胞用且没有暴露于600ns电脉冲而孵育亚10 nmαUnps。与我们的假设相反，发现NPSPEF暴露实际降低暴露细胞中的颗粒摄取。该结果表明，由于膜破坏，NPEF暴露可能会影响内吞途径和过程。

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