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Analysis of Microbial Community Structure in a Novel two-sludge Denitrifying Phosphorus Removal Process Using Fluorescence in Situ Hybridization (FISH)

机译:荧光原位杂交(FISH)分析新型二污泥脱氮除磷工艺中的微生物群落结构

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The microbial communities in a novel two-sludge denitrifying phosphorus removal process were investigated by usingrnFISH targeting the 16SrRNA of polyphosphate-accumulating organisms (PAO), ammonia-oxidizing bacteria (AOB) andrnnitrite-oxidizing bacteria (NOB), and DAPI chemical staining. The microbial morphology was also analyzed by SEM. It wasrnfound that PAO was effectively enriched in anoxic tank, occupying 46.7 of total bacteria. In nitrification tank, the numbers ofrnAOB were higher than that of NOB, accounting for 46.2% of total bacteria and 28.5% of that. There was a strong difference ofrnmicrobial morphologies between anoxic tank and nitrification tank. A variety of rod morphology microorganisms was found inrnanoxic tank, while nitrification sludge consisted of clusters of microorganisms. These results suggesting that the novelrntwo-sludge can provide a benefit for the cultivation of PAO or nitrifiers compared with the single sludge.
机译:通过使用针对多磷酸盐累积生物(PAO),氨氧化细菌(AOB)和亚硝酸盐氧化细菌(NOB)的16SrRNA的rnFISH以及DAPI化学染色,研究了新型的两污泥反硝化除磷工艺中的微生物群落。还通过SEM分析了微生物形态。结果发现,PAO在缺氧池中有效富集,占细菌总数的46.7。在硝化池中,rnAOB的数量高于NOB,分别占细菌总数的46.2%和28.5%。缺氧池和硝化池之间的微生物形态差异很大。在缺氧槽中发现了多种杆状微生物,而硝化污泥则由微生物簇组成。这些结果表明,与单一污泥相比,新型污泥可以为PAO或硝化剂的培养提供有益的条件。

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