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首页> 外文会议>European Symposium on Environmental Biotechnology(ESEB 2004); 20040425-20040428; Oostende; BE >Evaluation of alkane biodegradation potential of environmental samples by competitive PCR
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Evaluation of alkane biodegradation potential of environmental samples by competitive PCR

机译：通过竞争性PCR评价环境样品中烷烃的生物降解潜力

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摘要

A molecular method for evaluation of the biodegradation potential of microflorae of polluted sites was tested focusing on the detection of the alkB gene, encoding alkane hydroxylase which catalyses the hydroxylation of n-alkanes. AlkB-related genes are found in many bacterial genera, and often have reduced sequence similarities. Therefore, four different primer pairs were designed, based on classification of available partial sequences of alkane hydroxylase genes. These primers allowed amplification of alkB-related genes in 14 out of 15 tested alkane-degrading strains or microcosms. In addition, competitive PER experiments showed that alkB genes could be correctly quantified in the tested strains. AlkB genes were also quantified in pristine or contaminated water samples and compared to the corresponding n-heptane degradation rate. Results show that alkB gene copy numbers follow the same tendency as degradation rates and suggest the suitability of this method for a rapid evaluation of hydrocarbon degradation capacities of environmental samples.
机译：测试了一种评估污染部位微生物群落生物降解潜力的分子方法，重点是检测编码催化正构烷烃羟化的烷烃羟化酶的alkB基因。 AlkB相关基因存在于许多细菌属中，通常具有降低的序列相似性。因此，基于烷烃羟化酶基因的可用部分序列的分类，设计了四个不同的引物对。这些引物允许在15个测试的烷烃降解菌株或微观世界中的14个中扩增alkB相关基因。此外，竞争性PER实验表明，可以在测试菌株中正确定量alkB基因。还对原始或受污染的水样品中的AlkB基因进行了定量，并与相应的正庚烷降解率进行了比较。结果表明，alkB基因拷贝数遵循与降解率相同的趋势，并表明该方法适用于快速评估环境样品的烃降解能力。

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