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首页> 外文会议>European Symposium on Environmental Biotechnology(ESEB 2004); 20040425-20040428; Oostende; BE >Characterisation of three aerobic bacteria isolated from NPEO-contaminated sludges
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Characterisation of three aerobic bacteria isolated from NPEO-contaminated sludges

机译：从NPEO污染的污泥中分离出的三种需氧细菌的表征

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摘要

In this work, three aerobic bacteria were isolated from activated sludges contaminated by nonylphenol polyethoxylates and characterised. Two of them, namely BCaL1 and BCaL2 were Gram negative rods, the third, VA160, was a Gram positive bacterium with a rhizoid colony shape. Analysis of the 16S ribosomal DNAs showed that the three strains could be assigned to Acinetobacter (BCaL1) Stenotrophomonas (BCaL2) and Bacillus (VA160) genera. Their degradation ability towards commercial mixtures of nonylphenol ethoxy-lates, in particular towards a mixture having an average ethoxylation degree of five, was investigated in aerobic batch culture conditions. Results showed that the Acinetobacter and Stenotrophomonas strains were both able to degrade some components of the surfactant mixture. On the contrary Bacillus VA160 did not exhibit any detectable biodegradation activity, but, when co-cultured with the other two strains, it was capable of positively affecting their degradation ability.
机译：在这项工作中，从被壬基酚聚乙氧基化物污染的活性污泥中分离出了三个好氧细菌并进行了表征。其中的两个，即BCaL1和BCaL2是革兰氏阴性菌，第三个，VA160是具有根状菌落形状的革兰氏阳性菌。对16S核糖体DNA的分析表明，这三个菌株可以归为不动杆菌属（BCaL1）嗜单胞菌（BCaL2）和芽孢杆菌（VA160）属。在有氧分批培养条件下，研究了它们对壬基酚乙氧基化物商品混合物的降解能力，特别是对平均乙氧基化度为5的混合物的降解能力。结果表明，不动杆菌和嗜单胞菌都能够降解表面活性剂混合物的某些组分。相反，芽孢杆菌VA160没有表现出任何可检测的生物降解活性，但是当与其他两种菌株共培养时，它能够积极地影响其降解能力。

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