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首页> 外文会议>European Symposium on Environmental Biotechnology(ESEB 2004); 20040425-20040428; Oostende; BE >Sulfate reduction at low pH by a defined mixed culture of acidophilic bacteria
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Sulfate reduction at low pH by a defined mixed culture of acidophilic bacteria

机译：通过在酸性条件下进行嗜酸性细菌的混合培养来降低硫酸盐的含量

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摘要

Sulfidogenesis in a bench-scale bioreactor containing a mixed culture of acid-tolerant sulfate reducing bacteria (aSRB) and acidophilic heterotrophic bacteria was investigated. A 21 bioreactor was inoculated with Desulfosporosintts strain M1 and an Acidocella-like isolate (PFBC). PFBC was added to the reactor in order to remove acetic acid that was produced by the aSRB by incomplete oxidation of glycerol, and which is highly toxic at relatively low concentrations (often <500 μM) to most acidophiles. The bioreactor pH was maintained at 4.0 and glycerol was supplied as energy- and carbon-source. Zinc sulfate was also added to the bioreactor to remove sulfide generated by the aSRB as insoluble ZnS. Changes in concentrations of sulfate, zinc, glycerol and acetic acid were monitored, together with total cell counts. The relative abundance of Desulfosporosinus M1 and Acidocella PFBC was estimated using fluorescent in situ hybridization. The oxidation of glycerol was shown to be coupled to the reduction of sulfate. At the pH that the culture was maintained (pH 4.0), zinc was selectively precipitated as zinc sulfide while iron remained in solution. With this mixed culture, the concentrations of acetic acid were maintained at low levels (< 1 mM), presumably due to it being metabolized by the acetotrophic Acidocella PFBC. Previous attempts to demonstrate sulfidogenesis at low pH by pure cultures of SRB have proved unsuccessful. These results presented in this paper suggest that syntrophic mixed cultures of aSRB and acetotrophic microorganisms are effective at low pH, and may be used for selective removal of metals from acidic waste waters.
机译：在包含耐酸硫酸盐还原菌（aSRB）和嗜酸异养细菌的混合培养的台式生物反应器中，研究了硫的生成。用脱硫孢子菌菌株M1和酸球菌分离株（PFBC）接种21个生物反应器。将PFBC添加到反应器中是为了除去乙酸，该乙酸是由甘油不完全氧化而由aSRB产生的，并且在相对较低的浓度（通常<500μM）下对大多数嗜酸菌具有高毒性。生物反应器的pH值保持在4.0，甘油作为能源和碳源提供。硫酸锌也被添加到生物反应器中，以去除由aSRB生成的不溶性ZnS硫化物。监测硫酸盐，锌，甘油和乙酸浓度的变化，以及总细胞数。使用荧光原位杂交技术估算了脱硫孢子虫M1和嗜酸小球菌PFBC的相对丰度。已表明甘油的氧化与硫酸盐的还原有关。在维持培养物的pH（pH 4.0）下，锌以硫化锌的形式选择性沉淀，而铁保留在溶液中。在这种混合培养中，乙酸的浓度保持在较低的水平（<1 mM），这可能是由于乙酸是由乙酸营养性嗜酸菌PFBC代谢而得。先前通过纯SRB培养物证明在低pH条件下发生硫离子生成的尝试均未成功。本文提出的这些结果表明，aSRB和营养型微生物的营养混合培养物在低pH值下是有效的，可用于从酸性废水中选择性去除金属。

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