首页> 外文会议>Fourth Asian Fisheries Forum Beijing 16-20 October 1995 >Detection of Foreign DNA in Transgenic Fish with PCR technology
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Detection of Foreign DNA in Transgenic Fish with PCR technology

机译:PCR技术检测转基因鱼中的外源DNA

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摘要

Recently, the study of fish as a model system of transgenic animals develops rapidly. (3.47). The main reason is that transgenic manipulation on fish embryos is relatively easier. For example, just microinjection of foreign DNA into occyte plasma of fertilized fish eggs may bring a highly effective integration. in mammal, howeer, the integration does not occur unless foreign DNA is injected into procaryon of ossperm. The advantage of transqenic manipulation on fish not only opens a new fiel for gene engineering, but also provides a conventient tool for the sudy of regulating eukaryotic genes. With the development of the research, some new problems arise-how to detct foreign DNA conveniently. It is discovered that microiniected foreign DNA can duplicate themselves in embryos and the maintained until the late stage of embryo development.
机译:近年来,鱼类作为转基因动物模型系统的研究迅速发展。 (3.47)。主要原因是对鱼胚进行转基因操作相对容易。例如,仅将外源DNA微注射到受精鱼卵的卵细胞血浆中,就可以带来高度有效的整合。然而,在哺乳动物中,除非将外源DNA注入到胚乳的前体中,否则整合不会发生。对鱼类进行跨性别操作的优势不仅为基因工程开辟了一个新领域,而且为调节真核基因的研究提供了方便的工具。随着研究的发展,出现了一些新的问题-如何方便地检测外来DNA。发现微污染的外源DNA可以在胚胎中复制并维持到胚胎发育的后期。

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