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首页> 外文会议>High pressure food science, bioscience and chemistry >Effects of Pressure on Minor Groove Binding to DNA Studied by REPA and Fluorometric Methods
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Effects of Pressure on Minor Groove Binding to DNA Studied by REPA and Fluorometric Methods

机译：REPA和荧光法研究压力对小沟与DNA结合的影响

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As well characterized, perturbation by high hydrostatic pressure is a very useful tool to study the mechanisms of the interactions between biological molecules. For enzymes-substrates and protein-protein interactions, many examples have been reported in the series of the EHPRG meeting and other occasions. One of the key processes very important in the elucidation of the biological functions is the recognition of nucleic acid sequences and structures by low and high molecular weight substances, from antibiotics to regulation proteins, and rather limited number of examples of high pressure studies have been reported for the processes including nucleic acids. We have published that the high pressure preturbation resulted in a recovery of the relaxed sequence recognition by restriction endonucleases, caused by solvent or ionic strength perturbation, which implicated the strengthening of the enzyme-DNA interactions under high pressure.
机译：众所周知，高静水压扰动是研究生物分子之间相互作用机理的非常有用的工具。对于酶-底物和蛋白质-蛋白质的相互作用，在EHPRG会议系列及其他场合已报道了许多例子。在阐明生物学功能中非常重要的关键过程之一是通过低分子量和高分子量物质（从抗生素到调节蛋白）识别核酸序列和结构，并且已经报道了数量有限的高压研究实例用于包括核酸在内的过程。我们已经发布了高压扰动导致限制性核酸内切酶恢复了放松的序列识别，这是由溶剂或离子强度扰动引起的，这暗示着高压下酶-DNA相互作用的增强。

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