The human umbilical vein as a biologic scaffold for vocal fold reconstruction

机译：人脐静脉作为声带重建的生物支架

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A variety of extracellular matrix (ECM) scaffolds have been promising in various tissue engineering applications, such as the porcine small intestinal submucosa. Yet no particular scaffolds have been optimal for vocal fold regeneration. The decellularized human umbilical vein (HUV) is a novel allogeneic scaffold that has shown some promise for cardiovascular tissue engineering. This study examines the potential of the HUV as an acellular scaffold for engineering the vocal fold, in order to develop an implantable tissue substitute that can promote constructive tissue remodeling. A saline-based decellularization protocol was used to fabricate a biodegradable, acellular, three-dimensional scaffold from native HUV tissue. Histological examination and scanning electron microscopy demonstrated that native cellular materials in the HUV were removed with a fine three-dimensional structure of proteins and proteoglycans well preserved. Primary human vocal fold fibroblasts were seeded on the abluminal surface of the acellular HUV scaffold and cultured for 21 days. Significant proliferation and infiltration of the fibroblasts in the scaffold were observed. These data supported the promise of the HUV scaffold for vocal fold regeneration and restoration.

机译：各种细胞外基质（ECM）支架在各种组织工程应用中都有希望，例如猪小肠粘膜下层。然而，还没有特定的支架对于声带再生是最佳的。脱细胞的人脐静脉（HUV）是一种新型的异基因支架，已显示出对心血管组织工程的希望。这项研究检查了HUV作为脱细胞支架工程声带的潜力，以开发可促进建设性组织重塑的可植入组织替代物。基于盐水的脱细胞方案用于从天然HUV组织制备可生物降解的无细胞三维支架。组织学检查和扫描电子显微镜显示，HUV中的天然细胞物质被清除，并保留了良好的蛋白质和蛋白聚糖的精细三维结构。将主要的人声折叠成纤维细胞接种在脱细胞HUV支架的管腔表面上，并培养21天。观察到支架中成纤维细胞的显着增殖和浸润。这些数据支持了HUV支架在声带再生和恢复方面的前景。

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《IEEE Annual Northeast Bioengineering Conference》|2010年|P.1-2|共2页
会议地点 New York NY(US)
作者
Chan R. W.;
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Biomedical Engineering;

Otolaryngology - Head Neck Surgery University of Texas Southwestern Medical Center Dallas Texas 75390-9035;

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1. The human umbilical vein with Wharton's jelly as an allogeneic, acellular construct for vocal fold restoration. [J] . Chan RW, Rodriguez ML, McFetridge PS Tissue engineering, Part A . 2009,第11期

机译：沃顿氏胶冻为同种异体，无细胞结构的人脐带，用于声带修复。
2. Development of human umbilical vein endothelial cell (HUVEC) and human umbilical vein smooth muscle cell (HUVSMC) branch/stem structures on hydrogel layers via biological laser printing (BioLP) [J] . P K Wu, B R Ringeisen Biofabrication . 2010,第1期

机译：通过生物激光印刷（BioLP）在水凝胶层上开发人脐静脉内皮细胞（HUVEC）和人脐静脉平滑肌细胞（HUVSMC）分支/茎结构
3. Biological properties of crosslinked salmon collagen fibrillar gel as a scaffold for human umbilical vein endothelial cells. [J] . Nagai N, Mori K, Munekata M Journal of biomaterials applications . 2008,第3期

机译：交联的鲑鱼胶原原纤维凝胶作为人脐静脉内皮细胞支架的生物学特性。
4. The human umbilical vein as a biologic scaffold for vocal fold reconstruction [C] . R. W. Chan Annual Northeast Bioengineering Conference . 2010

机译：人类脐静脉作为声带重建的生物学支架
5. Human cytomegalovirus infection in human umbilical vein endothelial cells; human cytomegalovirus infection in hematopoietic progenitor cells: Model of HCMV latency [D] . Zhuravskya, Tatiana. 1997

机译：人脐静脉内皮细胞中的人巨细胞病毒感染；造血祖细胞中人类巨细胞病毒感染：HCMV潜伏期模型
6. The Human Umbilical Vein with Whartons Jelly as an Allogeneic Acellular Construct for Vocal Fold Restoration [O] . Roger W. Chan, Maritza L. Rodriguez, Peter S. McFetridge -1

机译：沃顿氏胶作为异源无细胞构建人声折叠的人脐静脉。
7. The Human Umbilical Vein with Wharton's Jelly as an Allogeneic, Acellular Construct for Vocal Fold Restoration [O] . Chan, Roger W., Rodriguez, Maritza L., McFetridge, Peter S. 2009

机译：沃顿氏胶作为异源，无细胞构建人声折叠的人脐静脉。

The human umbilical vein as a biologic scaffold for vocal fold reconstruction

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