• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>IEEE Annual Northeast Bioengineering Conference >Electrospun chitosan-based nanofiber scaffolds for cardiac tissue engineering applications
【24h】

Electrospun chitosan-based nanofiber scaffolds for cardiac tissue engineering applications

机译：基于静电纺丝壳聚糖的纳米纤维支架，用于心脏组织工程应用

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The objective of this study was to fabricate 3-dimensional (3D) chitosan nanofiber scaffolds using an electrospinning technique and explore its potential for cardiac tissue engineering. Three culture conditions were tested: cardiomyocytes only, cardiomyocytes-fibroblasts cocultures, and cardiomyocyte-endothelial cells cocultures. The cells were seeded on 2-dimensional (2D) chitosan films and 3D chitosan nanofibers. Cellular morphology and functionality were assessed using immunofluorescent staining for alpha-sarcomeric actin (SA) and gap junction protein, connexin-43 (Cx43). In both the 2D and 3D scaffolds, only the cardiomyocyte-fibroblasts cocultures resulted in polarized cardiomyocyte morphology, fibril SA expression and Cx43 expression was higher compared to the other two conditions. In addition, the fibroblasts cocultures demonstrated synchronized contractions involving large tissue-like cellular networks. To our knowledge, this is the first attempt to utilize 3D chitosan nanofibers as cardiomyocyte scaffolds. Our results demonstrate that chitosan nanofibers can serve as a potential scaffold that can retain cardiomyocyte morphology and function.
机译：这项研究的目的是使用静电纺丝技术制造3维（3D）壳聚糖纳米纤维支架，并探索其在心脏组织工程中的潜力。测试了三种培养条件：仅心肌细胞，心肌细胞-成纤维细胞共培养和心肌细胞-内皮细胞共培养。将细胞接种在二维（2D）壳聚糖膜和3D壳聚糖纳米纤维上。使用免疫荧光染色对α-肌节肌动蛋白（SA）和缝隙连接蛋白连接蛋白43（Cx43）进行细胞形态和功能评估。在2D和3D支架中，仅心肌细胞-成纤维细胞共培养导致极化的心肌细胞形态，与其他两个条件相比，原纤维SA表达和Cx43表达更高。此外，成纤维细胞共培养显示出同步收缩，涉及大型组织样细胞网络。据我们所知，这是将3D壳聚糖纳米纤维用作心肌细胞支架的首次尝试。我们的结果表明，壳聚糖纳米纤维可以作为潜在的支架，可以保留心肌细胞的形态和功能。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号