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首页> 外文会议>Imaging, manipulation, and analysis of biomolecules, cells, and tissues XII >Optical properties of Red Blood Cells - An optical tweezer based analysis
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Optical properties of Red Blood Cells - An optical tweezer based analysis

机译：红细胞的光学特性-基于光学镊子的分析

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A microscopic object finds an equilibrium orientation under a laser tweezer such that a maximum of its volume lies in the region of highest electric field. Furthermore, birefringent microscopic objects show no rotational diffusion after reorienting under a linearly polarized optical trap and also are seen to follow the plane of polarization when the latter is changed using a half wave plate. We observe that a healthy human Red Blood Cell (RBC) reproduces these observations in an optical tweezer, which confirms it to be birefringent. Polarization microscopy based measurements reveal that the birefringence is confined to the cell's dimple region and the mean value of retardation for polarized green light (λ = 546nm) is 9 ± 1.5nm. We provide a simple geometrical model that attributes the birefringence to the nature of arrangement of the phospholipid molecules of the bilayer. This predicts the observed variation in the measured birefringence, from the dimple to the rim of the cell which we further show, can serve to demarcate the extent of the dimple region. This points to the value of birefringence measurements in revealing cell membrane contours.. We extend this technique to understand the birefringence of a chicken RBC, an oblate shaped cell, wherein the slow axis is identified to be coincident with the long axis of the cell. Further, we observe the birefringence to be confined to the edges of the cell. Experiments to probe the optomechanical response of the chicken RBC are in progress.
机译：微观物体在激光镊子下找到平衡取向，从而其最大体积位于最高电场的区域中。此外，在线性偏振光阱下重新定向后，双折射微观物体没有旋转扩散，并且当使用半波片改变偏振平面时，还可以看到其遵循了偏振平面。我们观察到健康的人类红细胞（RBC）在光学镊子中再现了这些观察结果，这证实了它是双折射的。基于偏振显微镜的测量结果表明，双折射仅限于细胞的酒窝区域，偏振绿光（λ= 546nm）的延迟平均值为9±1.5nm。我们提供了一个简单的几何模型，该模型将双折射归因于双层磷脂分子的排列性质。这预测了所观察到的双折射的观察到的变化，从凹坑到细胞的边缘，我们将进一步展示这些变化可以用来划定凹坑区域的范围。这表明了双折射测量在揭示细胞膜轮廓方面的价值。我们扩展了这项技术，以了解鸡RBC（扁圆形细胞）的双折射，其中慢轴被确定为与细胞的长轴重合。此外，我们观察到双折射被限制在单元的边缘。鸡红细胞的光机械响应实验正在进行中。

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