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首页> 外文会议>Induced mutations and molecular techniques for crop improvement >THE SEARCH FOR MOLECULAR MARKERS LINKED TO ALUMINIUM TOLERANCE IN CEREALS
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THE SEARCH FOR MOLECULAR MARKERS LINKED TO ALUMINIUM TOLERANCE IN CEREALS

机译：与谷物中铝耐受性相关的分子标记的搜索

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Protein expression was examined in an isogenic system of wheat (Triticum aestivum L.) to determine if proteins can be used as molecular markers of Al tolerance. This system consisted of a dominant gene transferred from Maringa (Al tolerant) to Katepwa (Al sensitive) and was isolated in the near isoline Alikat (Al tolerant). Studies, including L-[~(35)S]-methionine incorporation, showed that protein synthesis increased in the root tips of all three lines. Katepwa showed a lack of protein accumulation in the root tips relative to the increased root tip dry weight with Al stress. This lack of protein accumulation was associated with an increase in proteinase activity in Al sensitive Katepwa. Maringa and Alikat showed normal, relative changes in the root tip dry weight and protein content and no increase in the proteinase activity following Al stress. Root tip polypeptide profiles showed that Al tolerance in Maringa, Alikat and Al tolerant F_2s was a constitutive response; there were marginal quantitative changes in the polypeptide profile following Al stress. In contrast, Katepwa showed numerous polypeptides that were either enhanced or reduced in quantity, following Al stress. It was concluded that changes in gene expression may be deleterious to Al tolerance in wheat and are associated with Al sensitivity. This isogenic system is also being used to isolate DNA markers that co-segregate with the Al tolerance phenotype. Two restriction fragment length polymorphism markers recently isolated by C. Riede at North Dakota State University, Fargo, United States of America, are located on chromosome 4DL. These markers are being mapped in an F_2 population derived from Katepwa × Alikat. The current status and progress of this research are discussed.
机译：在小麦的等基因系统（Triticum aestivum L.）中检查蛋白质表达，以确定蛋白质是否可以用作耐铝性的分子标记。该系统由从Maringa（耐铝）向Katepwa（铝敏感）转移的显性基因组成，并在近等位基因Alikat（耐铝）中分离。包括L- [〜（35）S]-蛋氨酸掺入在内的研究表明，蛋白质合成在所有三个品系的根尖均增加。 Katepwa显示，相对于Al胁迫，根尖干重增加，根尖中缺乏蛋白质积累。缺乏蛋白质积累与Al敏感的Katepwa中蛋白酶活性的增加有关。铝胁迫后，Maringa和Alikat的根尖干重和蛋白质含量显示出正常的相对变化，而蛋白酶活性没有增加。根尖多肽图谱表明，Maringa，Alikat和耐铝F_2s中的耐铝性是一种本构反应。在铝胁迫后，多肽谱有少量的定量变化。相反，在Al胁迫下，Katepwa显示出许多数量增加或减少的多肽。结论是基因表达的变化可能对小麦的耐铝性有害，并且与铝敏感性相关。该同基因系统还用于分离与Al耐受表型共分离的DNA标记。美利坚合众国法戈的北达科他州立大学的C. Riede最近分离出的两个限制性片段长度多态性标记位于染色体4DL上。这些标记被映射在源自Katepwa×Alikat的F_2种群中。讨论了这项研究的现状和进展。

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    D.J. SOMERS; K.G. BRIGGS; E. BUTLER; J.P. GUSTAFSON;

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    Plant Science Unit, Department of Agronomy University of Missouri, Columbia, Missouri, United States of America;

    Department of Agricultural, Food and Nutritional Science, Faculty of Agriculture, Forestry and Home Economics, University of Alberta, Edmonton, Alberta, Canada;

    Plant Science Unit, Department of Agronomy University of Missouri, Columbia, Missouri, United States of America;

    Plant Science Unit, Department of Agronomy University of Missouri, Columbia, Missouri, United States of America Plant Genetics Research Unit, Agricultural Research Service, United States Department of Agriculture University of Missouri, Columbia, Missouri, United States of America;

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  • 中图分类 作物遗传育种与良种繁育;
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